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Lactobacillus plantarum capable of relieving irritable bowel syndrome and application of lactobacillus plantarum

A technology for irritable bowel syndrome and Lactobacillus plantarum, applied in the field of cargo storage, can solve the problems of less choice and complex pathogenesis, and achieve the effects of reducing content, reducing activation, and improving flora imbalance

Pending Publication Date: 2022-03-18
UNIV OF SHANGHAI FOR SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The pathogenesis of this process is complex, the current drug treatment options are few, and there are certain side effects

Method used

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  • Lactobacillus plantarum capable of relieving irritable bowel syndrome and application of lactobacillus plantarum
  • Lactobacillus plantarum capable of relieving irritable bowel syndrome and application of lactobacillus plantarum
  • Lactobacillus plantarum capable of relieving irritable bowel syndrome and application of lactobacillus plantarum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 : Rat irritable bowel syndrome model establishment and experimental grouping

[0023] This example is the process of establishing a rat visceral hypersensitivity model and grouping experiments.

[0024] 1. Establishment of visceral hypersensitivity model in rats

[0025] The establishment of the visceral hypersensitivity model of rats in this example adopts the method of acetic acid enema plus restraint stress.

[0026] The basic operation of the acetic acid enema plus restraint stress method is as follows:

[0027] The selection standard of rats is Female Wistar rats, 180g, 5-6 weeks. The rats were acclimatized to the environment for 1 week without any treatment. After 1 week, they were weighed, randomly divided into groups and subjected to experimental operations of acetic acid enema and restraint stress.

[0028] For each experimental rat, 24 hours before the experiment, fasting, water, intraperitoneal injection of 10% chloral hydrate (300mg / kg), thr...

Embodiment 2

[0035] Embodiment 2: Lactobacillus plantarum AR495 Effects on Colon Sensitivity Scores of Wistar Rats

[0036] This example mainly investigates the effect of Lactobacillus plantarum AR495 on colon sensitivity of Wistar rats.

[0037] Rats were fasted for 12 hours before the experiment, and the rats were anesthetized, and an 8F urinary catheter with an air bag was inserted through the anus (insert the end of the air bag 7 cm into the anus, and fix it at the base of the tail of the rat at 1.0 cm outside the anus). , put the rats into a self-made transparent plastic bucket cage, they can only move back and forth, and cannot turn around, wait for the rats to stabilize for 30 minutes, and start the test after they are fully adapted. mL, 0.3mL, 0.4mL, 0.5mL, 0.6mL, each expansion lasts 5min with an interval of 30 seconds.

[0038] Each rat was equipped with two sets of silver needle electrodes, three in each group, implanted in the external oblique muscle, 2 cm higher than the ...

Embodiment 3

[0041] Example 3 : Effects of Lactobacillus plantarum AR495 on colon histomorphology in Wistar rats

[0042] This example examines the effect of Lactobacillus plantarum AR495 on the histological morphology of the colon of Wistar rats.

[0043] image 3 It is a photomicrograph of the colon tissue of the rat of Example 3 of the present invention. Among them, the photos of rat colon tissues in each group were stained with HE and taken under a ×200 microscope.

[0044] The symptom of IBS is that there is no obvious colonic inflammatory infiltration, so the colonic histological observation was carried out on the rats, and the results were as follows: image 3 As shown, the colonic mucosal morphology of the three groups of rats was normal, the epithelial structure and crypts were intact, rich in goblet cells, the villi were arranged tightly and neatly, there was no inflammatory cell infiltration or mucosal damage, and no obvious congestion and edema. The results showed that t...

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Abstract

The invention provides a lactobacillus plantarum capable of relieving irritable bowel syndrome, which is characterized in that the lactobacillus plantarum is an AR495 strain and has been preserved in China General Microbiological Culture Collection Center (CGMCC) on March 22, 2017, and the preservation number is CGMCC No.14004. The lactobacillus plantarum is a lactobacillus plantarum strain. The lactobacillus plantarum AR495 can reduce the content of tryptase in intestinal tracts, alleviate the activation of mast cells, inhibit the activation of PAR2-TRPV1 receptors and recover a neuropathic pain threshold value, so that the IBS symptom is effectively improved, and the AR495 also can regulate the homeostasis of intestinal flora and improve the condition of dysbacteriosis.

Description

technical field [0001] The invention belongs to the field of cargo storage, and in particular relates to a plant lactobacillus capable of alleviating irritable bowel syndrome and an application thereof. Background technique [0002] Irritable bowel syndrome (IBS) is a group of intestinal functional diseases mainly characterized by abdominal pain or discomfort, accompanied by changes in bowel habits and stool properties, and its incidence is about 5.8%-17.5% , the pathogenesis is unclear. The proposed pathogenesis include abnormal intestinal motility, abnormal visceral sensitivity, infection, brain-gut interaction, neuroimmune mechanism, and neuroimmune endocrine disorder mechanism. [0003] Visceral hypersensitivity (VH) is one of the important pathophysiological mechanisms of irritable bowel syndrome, manifested as increased sensitivity to painful stimuli, or discomfort to physiological stimuli below the pain threshold. Manifested as abdominal pain and abdominal discomfor...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C11/10C12R1/25
CPCA23C11/10A23C11/106A23V2400/169Y02A50/30
Inventor 艾连中夏永军王光强熊智强宋馨张汇杨昳津
Owner UNIV OF SHANGHAI FOR SCI & TECH
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