Traditional Chinese medicine microecological preparation for improving growth performance of ducks and application of traditional Chinese medicine microecological preparation
A technology of microecology and growth performance of traditional Chinese medicine, which is applied in the fields of application, medical preparations containing active ingredients, antibacterial drugs, etc., can solve problems such as difficulty in adapting to market demand, large residues of chemical feed additives, serious side effects, etc., to achieve Improve serum biochemical and antioxidant indicators, have significant antibacterial effect, and improve body weight
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Embodiment example 1
[0040] Implementation Case 1: Preparation of Traditional Chinese Medicine Microecological Preparations
[0041] 1. Preparation of Probiotic Seed Solution
[0042]Pick a small amount of Lactobacillus plantarum MⅢ from the solid medium with an inoculation loop and inoculate it in the MRS broth medium, pick a small amount of Bacillus amyloliquefaciens L2 and inoculate it in the NB broth medium, and place them in a constant temperature shaker at 37 °C , cultivated aerobically at 220 rpm to the stationary phase; inoculated the above-mentioned seed liquid cultivated to the stationary phase into the corresponding liquid medium, and cultivated to the stationary phase at 37 °C and 220 rpm to obtain the seeds of Lactobacillus plantarum MⅢ liquid and seed liquid of Bacillus amyloliquefaciens L2.
[0043] A small amount of Bifidobacterium bifidum and Clostridium butyricum were picked and inoculated in MRS and RCM liquid medium respectively, and cultured anaerobically at 37 ℃ until the st...
Embodiment example 2
[0047] Implementation case 2: Oxford cup antibacterial experiment of traditional Chinese medicine microecological preparations
[0048] Test drug:
[0049] Antibiotics: Chlortetracycline;
[0050] Commercially available probiotics: Boluohui;
[0051] Chinese medicinal probiotics: prepared according to the method of Example 1;
[0052] Chinese medicine mixture: the Chinese medicine formula of embodiment 1 is mixed and pulverized;
[0053] Probiotic mixture: Mix the four seed solutions of Lactobacillus plantarum, Bifidobacterium bifidum, Bacillus amyloliquefaciens L2, and Clostridium butyricum at a volume ratio of 2:2:1:1, and then inoculate the nutrient substrate (corn Starch: brown sugar: fructooligosaccharide: lysine: talcum powder mass ratio = 2:2:2:2:1), fermented in airtight at 37 ℃ for 48 h, air-dried and pulverized into bacterial powder.
[0054] Dilute four indicator bacteria, namely Salmonella, Staphylococcus epidermidis, Staphylococcus aureus and Escherichia coli ...
Embodiment example 3
[0056] Implementation case 3: Effects of traditional Chinese medicine microecological preparations on the growth performance, immune organ index and serum indicators of Cherry Valley duck
[0057] 1. Animal experiment group design
[0058] 200 healthy male Cherry Valley ducks with an average weight of (73.87 ± 2.67) g at 0 days were selected, randomly divided into 4 groups, 50 ducks in each group (5 replicates in each group, 10 ducks in each replicate), and each duck Weigh, mark and record. Blank control group: feed basal rations to Cherry Valley ducks for 35 days; traditional Chinese medicine probiotics group: feed basal rations with 0.2% traditional Chinese medicine probiotics to Cherry Valley ducks for 35 days; antibiotic group: feed basal rations to Cherry Valley ducks Feed the basal diet supplemented with 0.015% chlortetracycline for 35 days; commercially available traditional Chinese medicine probiotics group: feed the basal diet supplemented with 0.2% commercially avai...
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