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Compound fermented liquid feed and preparation method thereof

A technology of liquid feed and compound fermentation, applied in animal feed, animal feed, bacteria used in food preparation, etc., can solve the problems of high material ratio, large material loss, poor fermentation, etc., achieve high activity of probiotics, reduce material Loss, reduce the effect of fermentation failure

Pending Publication Date: 2022-03-18
WUHAN SUNHY BIOLOGICAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the problems of large material loss, high material ratio and poor fermentation in the prior art after feed fermentation

Method used

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  • Compound fermented liquid feed and preparation method thereof
  • Compound fermented liquid feed and preparation method thereof
  • Compound fermented liquid feed and preparation method thereof

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preparation example Construction

[0027] The present invention also provides a preparation method for the above-mentioned compound fermented liquid feed, comprising the following steps:

[0028] (1) inoculating the composite bacteria into the culture medium for activation and expanded cultivation, and preparing the seed liquid of the composite bacteria;

[0029] Specifically, the composite bacteria include Bacillus, Lactobacillus bulgaricus, Lactobacillus plantarum, and Bacillus coagulans;

[0030] Inoculate Bacillus subtilis in the culture medium under aseptic conditions, adjust the pH to 7.2-7.5, rotate at 220r / min, and culture at 35-37°C for 20-22h to obtain a viable bacterial content ≥ 3×10 10 The Bacillus subtilis seed liquid of cfu / ml;

[0031] Inoculate Lactobacillus bulgaricus and Lactobacillus plantarum in MRS medium under aseptic conditions, adjust the pH to 7.0-7.2, and culture at 37-45°C for 24-48h to obtain viable bacteria content ≥ 1×10 10 cfu / ml Lactobacillus bulgaricus seed liquid and live ba...

Embodiment 1

[0039] The present embodiment provides a compound fermented liquid feed, which is prepared through the following steps:

[0040] (1) Inoculate Bacillus subtilis in the medium under aseptic conditions, adjust the pH to 7.2, rotate at 220r / min, and cultivate at 35°C for 20h to obtain a viable bacterial content of 3×10 10 Bacillus subtilis seed liquid in cfu / ml; culture medium includes: peptone 10.0g / l, beef extract 3.0g / l, sodium chloride 5.0g / l, and the balance is distilled water;

[0041] Lactobacillus bulgaricus and Lactobacillus plantarum were respectively inoculated in MRS medium under aseptic conditions, the pH was adjusted to 7.0, and cultured at 37°C for 24 hours, respectively, the content of viable bacteria was 1×10 10 cfu / ml Lactobacillus bulgaricus seed liquid and live bacteria content is 3×10 10 Cfu / ml Lactobacillus plantarum seed solution; MRS medium includes peptone 10g / l, beef powder 5g / l, glucose 20g / l, yeast powder 4g / l, sodium acetate 5g / l, dipotassium hydrogen ...

Embodiment 2

[0054] The present embodiment provides a compound fermented liquid feed, which is prepared through the following steps:

[0055] (1) Bacillus subtilis was inoculated in the culture medium under aseptic conditions, the pH was adjusted to 7.2, the rotation speed was 220r / min, and cultured at 37°C for 22h, the content of viable bacteria was 3.5×10 10 Bacillus subtilis seed liquid in cfu / ml; culture medium includes: peptone 10.0g / l, beef extract 3.0g / l, sodium chloride 5.0g / l, and the balance is distilled water;

[0056] Lactobacillus bulgaricus and Lactobacillus plantarum were respectively inoculated in MRS medium under aseptic conditions, the pH was adjusted to 7.0, and cultured at 42°C for 36 hours, the live bacteria content was 1.5×10 10 cfu / ml Lactobacillus bulgaricus seed liquid and live bacteria content is 3×10 10 Cfu / ml Lactobacillus plantarum seed solution; MRS medium includes peptone 10g / l, beef powder 5g / l, glucose 20g / l, yeast powder 4g / l, sodium acetate 5g / l, dipotas...

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Abstract

The invention belongs to the technical field of fermented feeds, and particularly provides a compound fermented liquid feed and a preparation method thereof. The compound fermentation liquid feed provided by the invention is a novel antibiotic-free green feed, and can effectively inhibit the growth and reproduction of harmful bacteria in a feeding system, improve and maintain the balance of intestinal flora, improve the immunity and disease resistance, replace part of antibiotics, improve the quality of animal products, effectively improve the body type of pigs and increase economic benefits; according to the method for the compound fermentation of the liquid feed provided by the invention, the compound strain and the compound feed additive which are optimally combined are used in the liquid fermentation feed, so that the purposes of improving the composition of a feed raw material nutrient solution, shortening the fermentation time, reducing the material loss and improving the utilization rate of the raw materials are achieved. The method is simple and easy to implement, and the prepared liquid fermented feed is high in probiotic activity and has the effect of replacing part of antibiotics.

Description

technical field [0001] The invention belongs to the technical field of fermented feed, and in particular relates to a compound fermented liquid feed and a preparation method thereof. Background technique [0002] Fermented Liquid Feed (Fermented Liquid Feed, FLF), as a new type of feed, was first used in the Netherlands in the late 1980s. After that, Denmark, France, Sweden, Spain, Switzerland and other countries also began to use it. In recent years, liquid fermented feed has been widely used in Europe and has become a research hotspot. Liquid fermented feed can improve the digestibility of feed and improve the growth performance of pigs, especially in preventing diarrhea of ​​weaned piglets, increasing feed intake and daily gain. [0003] However, there are still some key technical issues to be solved in practical applications. For example, studies on the conversion rate of fermented liquid feed have shown that feeding fermented liquid feed to weaned piglets can increase ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K10/12A23K10/14A23K50/30A23L3/3499
CPCA23K10/12A23K10/14A23K50/30A23L3/3499A23V2400/123A23V2400/169
Inventor 毛玲陶敏周明杨雄振庄斐曹蕊刘文月詹志春周樱
Owner WUHAN SUNHY BIOLOGICAL
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