Method and system for predicting purity of natural killer cells

A technology of natural killer cells and purity, applied in the field of biomedicine, can solve the problems of less than 1% when low and the difference of NK purity, and achieve the effect of reducing waste and loss

Pending Publication Date: 2022-03-08
SHANGHAI TENTH PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the existence of individual differences, there are also large differences in the purity of NK cells cultured in vitro. When the purity of NK cells is high, it can reach 99%, and when it is low, it is less than 1%.

Method used

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  • Method and system for predicting purity of natural killer cells
  • Method and system for predicting purity of natural killer cells
  • Method and system for predicting purity of natural killer cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] This example is the establishment process of the method for predicting the purity of natural killer cells, and the prediction model is obtained through the following experiments and analysis verification.

[0040] 1. Experiment and data collection

[0041] experimental method : From January 2020 to June 2021, 51 patients who participated in the in vitro culture of NK cells in Fundamental Biotech were selected for the experiment. Among them, there were 17 males and 34 females; the age ranged from 36 to 69 years, with an average age of 53 years. Before culturing NK cells in vitro, we tested the patients for a total of 138 indicators including blood routine, urine routine, biochemical routine, coagulation function, lymphocyte subsets, thyroid function, five items of hepatitis B and tumor indicators. According to the GMP production standard, NK cells are cultured by factor stimulation method, and then CD3 and CD56 molecules are detected on NK cells by flow cytometry, and...

Embodiment 2

[0068] This embodiment provides a method for predicting the purity of natural killer cells, the specific process is:

[0069] 1) Obtaining the red blood cell distribution width standard deviation of the target subject's peripheral blood by detecting the peripheral blood;

[0070] 2) Judging the level of natural killer cell purity based on the predictive relationship model between the natural killer cell purity and the red blood cell distribution width standard deviation, including the following predictive relationship formula: prediction index=-85.279+3.033*red blood cell distribution width standard deviation;

[0071] When the prediction index is greater than 45, it is judged that the purity of the natural killer cells is high;

[0072] When the prediction index is less than or equal to 45, it is judged that the purity of the natural killer cells is low;

[0073] Wherein, high purity means that the purity is greater than 45%; said low purity means that the purity is less tha...

Embodiment 3

[0075] This embodiment provides a system for predicting the purity of natural killer cells, the specific structure is:

[0076] 1) a detection module, used to detect the standard deviation of red blood cell distribution width in the peripheral blood of the target object;

[0077] 2) A prediction module, for predicting the purity of the natural killer cells based on the prediction relationship between the purity of the natural killer cells and the standard deviation of the red blood cell distribution width, including the following prediction models:

[0078] Prediction index=-85.279+3.033×RBC distribution width standard deviation;

[0079] When the prediction index is greater than 45, it is judged that the purity of the natural killer cells is high;

[0080] When the prediction index is less than or equal to 45, it is judged that the purity of the natural killer cells is low;

[0081] Wherein, high purity means that the purity is greater than 45%; said low purity means that t...

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Abstract

The invention discloses a method and a system for predicting the purity of natural killer cells. The method for predicting the purity of the natural killer cells comprises the following steps: acquiring a red blood cell distribution width standard deviation of peripheral blood of a target object; and determining the natural killer cell purity based on a prediction relationship between the natural killer cell purity and the red blood cell distribution width standard deviation. The method can effectively predict the cell purity of in-vitro culture of the natural killer cells of a target object, has sensitivity and specificity, can be used for judging the purity of the NK cells, and has extremely high clinical application value.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a method and system for predicting the purity of natural killer cells. Background technique [0002] Natural killer cells (Natural killer cells, NK cells) are lymphocytes of the innate immune system, and the cell surface contains a series of inhibitory receptors (CD94 / NKG2A), activating receptors (such as NKG2D) and natural cytotoxicity receptors (such as NKp46 , NKp44 and NKp30), so NK cells can recognize and kill diseased cells through surface receptors without prior sensitization. Twenty years ago, NK cell-mediated immunotherapy achieved a therapeutic breakthrough in patients with advanced leukemia, and the field of NK cell-based cancer therapy developed rapidly. [0003] NK cell-based immunotherapy includes NK cell adoptive immunotherapy, the use of immune checkpoint inhibitors, and the application of CAR-modified NK cells, etc. These immunotherapies provide more ther...

Claims

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Application Information

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IPC IPC(8): C12N5/078C12N5/0783C12Q1/02
CPCC12N5/0646C12N5/0641G01N33/5005Y02A50/30
Inventor 刘淑鹏程忠平葛瑶王春燕刘琦郭静熊兵
Owner SHANGHAI TENTH PEOPLES HOSPITAL
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