Full-automatic NGS library construction system

Abstract

The invention discloses a full-automatic NGS library construction system. Comprising a shell, a suction pipe arranged in the shell, a suction head arranged below the suction pipe, a pressing structure arranged below the shell, a connecting structure arranged in the shell, located below the shell and used for sealing the suction head, and a cleaning structure arranged in the shell and used for driving the connecting structure and cleaning the suction head. The heating structure is arranged in the shell, is positioned at the uppermost part and is used for providing air for the cleaning structure; by arranging the connecting structure, the sealing effect between the suction head and the suction pipe is improved, so that the shell can more accurately control the suction head to suck a solvent with a specified metering, and the detection accuracy is improved; a heating structure and a cleaning structure are arranged, the sealing effect is further improved, meanwhile, the connecting position of the connecting disc and the telescopic disc is cleaned, the matching effect between the connecting disc and the telescopic disc is further improved, and the effect of sucking the specified metering solvent is better.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid extraction, and in particular relates to a fully automatic NGS library construction system. Background technique [0002] The fully automatic NGS library construction system mainly includes nucleic acid extraction, PCR amplification, and library construction. The existing nucleic acid extraction method is mainly to install the tip on the mobile platform, and then absorb the solvent in the test tube and transport it To another test tube, this operation needs to be carried out several times. After each operation, some sterilized liquid is sucked into the tip, the tip is sterilized, and then the tip is used for many times, but this method will cause suction The head is not suitable for cleaning, and the cleaning time is short, which may lead to unclean cleaning, and then the connection between the suction head and the mobile platform is not tight, mainly through the docking between the two, but ...

AI Technical Summary

Problems solved by technology

[0002] The fully automatic NGS library construction system mainly includes nucleic acid extraction, PCR amplification, and library construction. The existing nucleic acid extraction method is mainly to install the tip on the mobile platform, and then absorb the solvent in the test tube and transport it To another test tube, this operation needs to be carried out several times. After each operation, some sterilized liquid is sucked into the tip, the tip is sterilized, and then the tip is used for many times, but this method will cause suction The head is not suitable for cleaning, and the cleaning time is short, which may lead to unclean cleaning, and then the connection between the suction head and the mobile platform is not tight, mainly through the docking between the two, but the sealing effect of the docking is not very ideal , so that there will be air leakage due to poor sealing, which will lead to inaccurate measurement of the inhaled solution, which is not conducive to the accuracy of detection after nucleic acid extraction, and inaccurate measurement of nucleic acid extraction

Images