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Application of felotinib in inhibition of proliferation of pancreatic cancer cells and tumor tissues

A technology for the proliferation of pancreatic cancer cells and tumors, applied in the field of drug screening for pancreatic cancer treatment, can solve the problems of increasing incidence of pancreatic cancer and limited therapeutic effect

Pending Publication Date: 2022-02-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite these advances and some improvements in overall survival for patients, pancreatic cancer is still a deadly disease with rising incidence worldwide and limited treatment outcomes

Method used

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  • Application of felotinib in inhibition of proliferation of pancreatic cancer cells and tumor tissues
  • Application of felotinib in inhibition of proliferation of pancreatic cancer cells and tumor tissues

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Example 1: Felotinib can inhibit the proliferation of pancreatic cancer cells (MIA PaCa-2)

[0018] 1. CCK8 experiment

[0019] MIA PaCa-2 cells were seeded in a 96-well cell culture plate, 100 μL of cell suspension per well, the number of cells was about 4000-5000 per well, and three replicate wells were made for each treatment concentration. Cultured in a 37°C incubator. After the cells adhered to the wall, they were treated with different concentrations of felotinib (0, 12.5, 25, 50 and 100 μmol / L) for 24 and 48 hours, and then cultured in serum-free culture with 10% CCK8. The base was added to the culture well in the form of changing the liquid, and cultured in a 37°C incubator for 2h, and the absorbance was detected at a wavelength of 450nm.

[0020] After 24 hours of felotinib treatment of MIA PaCa-2 cells, the detection results were as follows: figure 1 As shown, the results showed that the inhibitory rates of felotinib at concentrations of 12.5, 25, 50 and 100...

Embodiment 2

[0021] Example 2: Felotinib Can Inhibit Pancreatic Cancer Tumor Proliferation in Mice

[0022] 1. Xenograft mouse model

[0023] Animal experiments were carried out in accordance with the institutional guidelines for the care and use of experimental animals. Six 4-week-old female BALB / c nude mice were obtained from the Shanghai Experimental Animal Center of the Chinese Academy of Sciences. By injecting 1 × 10 in the mouse axilla under 3% isoflurane anesthesia 6 Luciferase-labeled MIA PaCa-2 cells (Luc-MIA PaCa-2) were used to establish a subcutaneous xenograft mouse model. Divide the mice into two groups of three mice each. The mice in the treatment group were injected intraperitoneally with 0.1 mL of felotinib (4.7 μmol / kg body weight) every other day. The control group was injected intraperitoneally with 0.1 mL DMSO. The mouse tumors were removed and weighed.

[0024] The result is as figure 2 As shown, the tumor size of mice treated with feilotinib was significantly ...

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Abstract

The invention provides application of felotinib in inhibition of tumor proliferation of pancreatic cancer cells and mouse pancreatic cancer, namely application in preparation of products for inhibition of tumor proliferation of pancreatic cancer cells or mouse pancreatic cancer. The invention further provides a product for inhibiting tumor proliferation of adenocarcinoma cells or mouse pancreatic cancer. The product contains the felotinib with pharmacological effective concentration. The effective pharmacological concentration is not less than 12.5 [mu]mol / L; and the product is a liquid product. The invention finds that the non-lotinib can inhibit the proliferation of pancreatic cancer cells and mouse pancreatic cancer tumors, and the non-lotinib can be used for preparing a novel medicine for treating pancreatic cancer.

Description

technical field [0001] The invention belongs to the technical field of drug screening for treating pancreatic cancer, and in particular relates to the application of Filgotinib in inhibiting the proliferation of pancreatic cancer cells and tumor tissues. Background technique [0002] Pancreatic cancer (PDAC) is a common malignant tumor of the digestive system with a very poor prognosis. It is one of the most aggressive tumors. As a result, clinical diagnosis and treatment are very difficult, and the five-year survival rate continues to rank the last among all tumors, far lower than other tumors. Today, the treatment of pancreatic cancer has advanced significantly, including surgical techniques and adjuvant and neoadjuvant therapies, which are increasingly used. Despite these advances, which have extended overall survival to some extent, pancreatic cancer remains a deadly disease with rising incidence worldwide and limited therapeutic efficacy. Therefore, new treatments are...

Claims

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Application Information

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IPC IPC(8): A61K31/541A61P35/00A61P1/18
CPCA61K31/541A61P35/00A61P1/18
Inventor 曹利平贾盛楠赵冰冰丁国平章朝雷
Owner ZHEJIANG UNIV
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