Biomarker genes for detecting colorectal cancer metastasis, application, kit and pharmaceutical composition

A technology of biomarkers and colorectal cancer, applied in the direction of drug combination, biochemical equipment and methods, microbiological determination/testing, etc., can solve the problem of few research reports on key regulatory genes, providing biomarkers, and no clinical diagnosis and treatment And other issues

Pending Publication Date: 2022-02-01
KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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  • Claims
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Problems solved by technology

However, there are few reports on the key regulatory genes involved in the process of colon cancer non-metastasis to metastasis, and there are no guiding biomarkers for clinical diagnosis and treatment.

Method used

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  • Biomarker genes for detecting colorectal cancer metastasis, application, kit and pharmaceutical composition
  • Biomarker genes for detecting colorectal cancer metastasis, application, kit and pharmaceutical composition
  • Biomarker genes for detecting colorectal cancer metastasis, application, kit and pharmaceutical composition

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Experimental program
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Embodiment 1

[0050] 1. Acquisition of biomarker genes

[0051] The clinicopathological data analyzed in this embodiment are obtained from the NCBI (National Center for Biotechnology Information) gene expression profile database (Gene Expression Omnibus (GEO) database, http: / / www.ncbi.nlm.nih.gov / geo / ) Six data sets GSE9348, GSE8671, GSE47908, GSE62932, GSE29623 and GSE40967 in GPL570 were obtained. By integrating the data in the dataset, 681 samples ( figure 1 ), more than 45,000 gene expression profile data, including 69 normal samples; 612 colorectal cancer samples (339 non-metastatic cases, 273 metastatic cases (78 cases of metastases were distant metastases), classified according to pathological features for follow-up Perform WGCNA analysis (Weighted Gene Co-Expression Network Analysis, weighted gene co-expression network analysis), the specific WGCNA analysis process is as follows figure 2 shown.

[0052] Using the dynamic tree cutting algorithm to identify co-expression modules r...

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Abstract

The invention provides a group of biomarker genes for detecting colorectal cancer metastasis, application, a kit and a pharmaceutical composition, and relates to the technical field of biomedical clinical diagnosis. The abnormal expression levels of the biomarker genes BGN, THBS2, SPARC, CDH11, MFAP2, MMP11, SPP1 and THY1 disclosed by the invention are obviously related to the colon cancer metastasis. In the embodiment of the invention, 80 clinical samples are adopted for immunohistochemical staining analysis of the expression conditions of the 8 genes in the clinical samples, then the expression quantity of the corresponding genes in the colon cancer cell line is reduced through the sh-RNA, and transwell cell chamber experiments verify that when the expression level of the target gene is inhibited, the invasion ability of the cell line is weakened. Therefore, the eight genes can be used as biomarkers of colon cancer metastasis, can be used as potential targets for treating colon cancer metastasis, and have important clinical application value.

Description

technical field [0001] The invention belongs to the technical field of biomedical clinical diagnosis, and specifically relates to a group of biomarker genes for detecting colorectal cancer metastasis, its application, a kit and a pharmaceutical composition. Background technique [0002] Colorectal cancer (CRC) is one of the most common malignant tumors in the world. According to the latest epidemiological statistics in 2020, among all cancer types, the incidence of colorectal cancer ranks among all age groups and genders. Third, and its fatality rate ranks second. [0003] Because the main symptoms of colorectal cancer in the early stage are not obvious, most of the patients are in the middle and late stages when they seek treatment. Some of them have missed the best time for surgical treatment when they are diagnosed, and can only be treated with radiotherapy and chemotherapy, which makes the prognosis of advanced colorectal cancer relatively poor. Poor, the case fatality ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61K31/713A61P35/00
CPCC12Q1/6886A61K45/00A61K31/713A61P35/00C12Q2600/158C12Q2600/118C12Q2600/136
Inventor 刘树柏何智承何英英
Owner KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
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