Preparation method of peony stamen and pistil protein powder
A peony stamen and protein powder technology, which is applied in the field of food processing, can solve the problems of not improving the solubility and emulsification of peony stamen protein at the same time, and being unable to expand the application range of peony stamens, etc.
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Embodiment 1
[0035] The preparation method of the peony stamen protein powder of the present embodiment comprises the following steps:
[0036] (1) Select fresh peony stamens that retain the pollen-like substances on peony stamens as raw materials, mechanically crush them through an 80-mesh sieve to obtain peony stamen powder, and mix peony stamen powder and petroleum ether at a ratio of 1:5 (g / mL) at room temperature The ratio was mixed to remove lipid substances, and the degreased powder was obtained after natural air drying, which was sealed and stored at 4°C for later use.
[0037] (2) Mix the degreased powder and deionized water in step (1) at a ratio of 1:20 (g / mL) to obtain a mixed solution, first add 0.1mol / L of sodium hydroxide to adjust the pH of the mixed solution to 9.0, Then heated in a water bath at 30° C. for 3 h, centrifuged at 4000 r / min for 20 min after the reaction, and collected the supernatant (ie, alkaline extraction solution) for later use.
[0038] (3) With the alkal...
Embodiment 2
[0044] The preparation method of the peony stamen protein powder of the present embodiment comprises the following steps:
[0045] (1) Select fresh peony stamens that retain the pollen-like substances on peony stamens as raw materials, mechanically crush them through a 90-mesh sieve to obtain peony stamen powder, and mix peony stamen powder and petroleum ether at a ratio of 1:6.5 (g / mL) at room temperature The ratio was mixed to remove lipid substances, and the degreased powder was obtained after natural air drying, which was sealed and stored at 4°C for later use.
[0046] (2) Mix the degreased powder and deionized water in step (1) at a ratio of 1:25 (g / mL) to obtain a mixed solution, first add 0.25mol / L of sodium hydroxide to adjust the pH of the mixed solution to 9.3, Then heated in a water bath at 40° C. for 2 h, centrifuged at 6000 r / min for 15 min after the reaction, and collected the supernatant (ie, alkaline extraction solution) for future use.
[0047] (3) With the ...
Embodiment 3
[0053] The preparation method of the peony stamen protein powder of the present embodiment comprises the following steps:
[0054] (1) Select fresh peony stamens that retain the pollen-like substances on peony stamens as raw materials, mechanically crush them through a 100-mesh sieve to obtain peony stamen powder, and mix peony stamen powder and petroleum ether at a ratio of 1:8 (g / mL) at room temperature The ratio was mixed to remove lipid substances, and the degreased powder was obtained after natural air drying, which was sealed and stored at 4°C for later use.
[0055] (2) Mix the degreased powder and deionized water in step (1) at a ratio of 1:30 (g / mL) to obtain a mixed solution, first add 0.5mol / L of sodium hydroxide, and then adjust the pH of the mixed solution to 9.5 , and then heated in a water bath at 50° C. for 1 h, centrifuged at 8000 r / min for 10 min after the reaction, and collected the supernatant (ie, alkaline extraction solution) for subsequent use.
[0056]...
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