Culture medium for rejuvenating edible fungus strain and method for rejuvenating edible fungus strain
A technology for cultivating substrates and edible fungi is applied in the field of agricultural biology, which can solve the problems of slow feeding of mycelium, weak mycelium of shiitake mushrooms, and easy early fruiting of culturing materials.
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Embodiment 1
[0036] 1) in a test tube was added 2ml of water agar (15g agar, 1000ml deionized water), placed vertically, autoclaving, sterilization temperature 115 deg.] C, sterilization time 15min, the test tube is placed vertically sterilized cool down.
[0037] 2) The specifications of 6cm × 0.5cm × 0.3cm hardwood strips soaked in deionized water three days, and then sterilized under conditions of 126 deg.] C 120min.
[0038] 3) Inoculation of mushroom strains on the cooled agar medium, then insert the sterilized hardwood wood inserted into the agar medium, place the test tube into the incubator (25 ° C) vertically.
[0039] 4) Through the bacterium to 3-4cm on the wood strip, starting a continuous passage training on the wooden strip.
Embodiment 2
[0052] The difference from Example 1 is only that the hardwood wood is replaced with a cork wood strip of the same specification.
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