Method for preparing fuel ethanol from corn straws
A technology of corn stalks and fuel ethanol, applied in the direction of microorganism-based methods, biochemical equipment and methods, biofuels, etc., can solve the problem that the conversion rate is not enough to meet industrial production, and achieve the effect of improving the conversion rate of sugar alcohol
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[0030] A method for producing powdered ethanol with corn stalks as a raw material, the specific steps comprising:
[0031] 1) plate culture
[0032] The composition of the plate medium is 1L of straw enzymatic hydrolysis solution (containing 3% glucose), accounting for 20% of the total weight, and 0.3% of Angelica thermostable yeast; the Angelica thermostable yeast is inserted into the medium, and cultured at 30°C 25 hours;
[0033] 2) Shake flask seed culture
[0034] The shake flask seed culture consists of 3% glucose, 0.2% yeast, 0.05% potassium dihydrogen phosphate, 0.06% ammonium sulfate, pH5.6, and tap water;
[0035] Inoculate the plate-cultured angel high-temperature-resistant yeast into the seed medium of the shaker flask, fill a 500ml Erlenmeyer flask with a liquid volume of 100ml, and cultivate it at 30°C for 32 hours, and the shaker speed is 200rpm;
[0036] 3) Seed pot seed culture
[0037] The composition of the seed tank seed medium is 22% of straw enzymatic...
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