Preparation and storage method and application of dry szechuan lovage rhizome asexual propagation szechuan lovage rhizome seeds
A technology of asexual reproduction and Chuanxiong Ling, applied in the application, seed and rhizome treatment, agriculture and other directions, can solve the problem of long-term effective preservation of Chuanxiong germplasm resources, etc., and achieve the effect of high storage and transportation costs.
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Embodiment 1
[0030] The growth vigor and vigor of Chuanxiong Rhizoma Chuanxiong asexually propagated seeds with different water contents are measured, and the specific implementation method is as follows:
[0031] S1: Harvesting of Rhizoma Chuanxiong stalks: During the ripening season of Rhizoma Chuanxiong Lingzhong (June to July), choose cloudy or sunny days after the dew dries up, pick the mature and strong stems of Ligusticum Chuanxiong, remove the top stems and leaves and bottom rhizomes, and thresh Harvest in bundles, and remove weak stems such as skewers, fennel stalks, Dadangdang, Yuzui and Begonia Ling. On the day of harvest, they will be transported to a cool and ventilated place indoors by dry and pollution-free means of transport.
[0032] S2: Treatment of Ligusticum chuanxiong Lingzhong: cut the rhizoma chuanxiong stalks into 3-4cm short nodes, leave an enlarged node disk in the middle of each node, and leave 1-2cm stems at both ends of the node disk. Spread the cut Ling seeds...
Embodiment 2
[0038] The storage time of Rhizoma Chuanxiong asexually propagated seed Lingzhong is tested under different temperatures, and the specific implementation method is as follows:
[0039] S1: Storage of dried Rhizoma Chuanxiong Lingzhong at different temperatures: Take dried Lingzhong with 10% water content as an example. The dried vegetative propagation seeds of Rhizoma Chuanxiong prepared by the method of the present invention are grouped into sealed bags, and three groups are stored in refrigerators at 4°C, -20°C and -80°C respectively, and one group is placed in liquid nitrogen at -196°C. Store in middle temperature, and store the remaining group at room temperature (about 25°C). After 14 days, the 5 groups of seeds were taken out and returned to room temperature for viability determination, and the test results are shown in Table 2.
[0040]S2: Determination of the vigor of Rhizoma Chuanxionglingling seeds after storage at different temperatures: Take out the sealed bags of...
Embodiment 3
[0044] The mature stem nodes of crops such as tomato, sweet potato and potato were used as materials to prepare vegetatively propagated seeds, and they were broadcast live. The development of the seeds is shown in the attached image 3 as shown in a. The stem nodes of fresh tomato and sweet potato have strong regenerative ability, and can take root and sprout; but the regenerative ability of stem nodes and roots of fresh potato is weak, and the roots still cannot regenerate after bud regeneration consumes the storage materials, and finally the stem nodes cannot regenerate into complete plants.
[0045] Get the vegetative propagation seeds of tomato and sweet potato in addition and dry to water content 10%, carry out sowing then, seed development situation is as attached image 3 as shown in b. The vegetative propagation seeds obtained from the stem nodes with a water content of 10% of tomato and sweet potato were not vigorous, and both could not take root and germinate normal...
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