Bacillus subtilis, phaffia rhodozyma and rhizopus mixed tobacco fermentation method

Abstract

The invention relates to a mixed tobacco fermentation method. The method comprises the following steps of respectively inoculating bacillus subtilis and phaffia rhodozyma on an LB agar culture medium and a YPD agar culture medium, respectively culturing for 24 hours at 37 DEG C and culturing for 5-7 days at 22 DEG C to obtain single colonies of the bacillus subtilis and the phaffia rhodozyma; respectively inoculating the single colonies into an LB liquid culture medium and a YPD liquid culture medium, respectively culturing for 20 hours at 37 DEG C and culturing for 28-29 hours at 22 DEG C to obtain a bacillus subtilis seed solution and a phaffia rhodozyma seed solution, and mixing the bacillus subtilis seed solution and the phaffia rhodozyma seed solution in an isopyknic manner to obtain a mixed bacterium solution; centrifuging the mixed bacterial liquid, collecting thalli and washing; resuspending the washed thalli to obtain a bacterial suspension; and adding the bacterial suspension and rhizopus powder into tobacco leaves, and fermenting at 25 DEG C for 7-10 days. By means of the method, the bacillus subtilis, the phaffia rhodozyma and the rhizopus are compounded and mixed, so that macromolecules such as starch and protein which have negative effects on the quality of the tobacco leaves can be degraded into alcohols, lipids, aldehydes and aroma small molecular substances, and the quality of the tobacco leaves is effectively improved.

Description

technical field [0001] The invention relates to a method for fermenting tobacco mixed with bacillus subtilis, Phaffia rhodozyme and rhizopus, belonging to the technical field of microbes. Background technique [0002] Tobacco leaf fermentation is an important method to improve the quality of tobacco leaves under natural or artificial intervention conditions after tobacco leaves are modulated. Although the sensory quality of the modulated tobacco leaves has been improved, it cannot be directly used to produce cigarettes because of its high irritation, heavy green miscellaneous odor, monotonous aroma, and insufficient aroma. Fermenting tobacco leaves with microorganisms can significantly improve the above defects and improve the quality of cigarettes. At the same time, in order to meet the requirement that heat-not-burn cigarettes can release more flavor substances at low temperatures, it can not only reduce the harm caused by the ignition and combustion of ordinary cigarette...

AI Technical Summary

Problems solved by technology

Although the sensory quality of the modulated tobacco leaves has been improved, it cannot be directly used to produce cigarettes due to its high irritation, heavy green odor, monotonous aroma, and insufficient aroma.

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