ELISA detection method for detecting human serum candida glabrata enolase IgG antibody

Abstract

The invention discloses an ELISA detection method for detecting a human serum candida glabrata enolase IgG antibody. The ELISA detection method comprises the following steps of: balancing each reagent to room temperature of 20-25 DEG C; taking 100 microliters of sample diluent as blank control, and adding the sample diluent into a blank hole of a coated enzyme-linked plate; taking 100 microliters of negative serum as a negative control, and adding the negative serum into a negative control hole of the coated enzyme-linked plate; taking 100 microliters of positive serum as a positive control, and adding the positive control into a positive control hole of the coated enzyme-linked plate; adding 100 microliters of serum to be detected diluted according to the ratio of 1: 500 into a detection hole; incubating at 37 DEG C for 60 minutes; performing washing; adding 100 microliters of an enzyme-labeled antibody into a hole of the coated enzyme-linked plate, and placing the coated enzyme-linked plate at 37 DEG C for 30 min; performing washing; performing color development; terminating the reaction; and detecting by using a microplate reader, setting zero by using the blank hole, reading a light absorption value under the wavelength of 450 nm, judging the effectiveness of the test, and calculating an S/N value. According to the method of the present invention, the candida glabrata enolase is adopted as the antigen, the indirect ELISA detection technology of the human serum candida glabrata enolase IgG antibody is established, the detection specificity reaches 100%; and the ELISA detection kit has advantages of high sensitivity and good inter-batch stability.

Description

technical field [0001] The invention relates to an ELISA detection method for detecting human serum candida glabrata enolase IgG antibody, belonging to the technical field of biology. Background technique [0002] Candida is a normal parasitic flora of the human body, but when certain factors disrupt the balance between the host’s immune system and Candida, Candida can invade host tissues and blood, and grow and reproduce in them, leading to tissue damage, organ dysfunction and Pathological changes such as inflammatory response, such infection is invasive candidiasis (IC). Currently, clinical medicine is facing more and more challenges of IC, and the incidence of IC continues to rise in many regions. Even if patients receive antifungal treatment, the mortality rate is as high as 40%. Candida albicans is the most common pathogen causing IC in most clinical cases. In recent years, other non-albicans species have accounted for an increasing proportion of IC cases due to the u...

AI Technical Summary

Problems solved by technology

However, the biggest problem in the field of IC diagnosis is that the research investment in the detection of non-albicans infection is seriously insufficient, and the serological diagnosis technology of Candida glabrata infection is still blank.

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