Molecular mechanism research method for anthocyanin accumulation in pakchoi based on UV-A coupling
A UV-A, pakchoi technology, applied in the field of light regulation, can solve the problems of lack of metabolism level, gene level, etc.
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Embodiment 1
[0034]Using pak choy as an experimental raw material, the method for carrying out photoregulation and anthocyanin content measurement of pak choy comprises the following steps:
[0035] S1. Select the pakchoi seeds required for the experiment, and select excellent pakchoi seeds with high survival rate, then soak the seeds in sterile water, then embellish them on the germination paper, transfer them to the dark for accelerated germination, Then select the dew-white seeds and sow them on the moist sponge block to carry out the work of raising seedlings;
[0036] S2. For the pakchoi seedlings cultivated in step S1, select robust and consistent seedlings to be fixed in the hydroponic box, and artificially intervene in the temperature and humidity of the seedling room and the climate chamber. The light treatments tested in the experiment are different light treatments. Strong UV-A and blue light;
[0037] S3. For the pakchoi seedlings cultivated in step S1, deploy the nutrient ele...
Embodiment 2
[0049] The method of photoregulation and anthocyanin content measurement of pakchoi provided in this example is roughly the same as that of Example 1, the main difference is that in step S1, the soaking time in sterile water is 30 minutes, and the dark germination time is 24 hours;
[0050] In step S2, the temperature in the nursery room and the artificial climate chamber is 25° C., and the humidity is 85° C.
[0051] The photoperiod in step S2 is ten hours per day.
[0052] The nutrient solution prepared in step S3 is made of potassium nitrate, calcium nitrate, ammonium dihydrogen phosphate and magnesium sulfate, wherein the concentration of soluble ions is 1.3ds / m, and the pH is 6.0.
[0053] In step S5, the centrifugal temperature of the centrifuge is 4° C., the rotational speed of the centrifuge is 12000 rpm, and the separation time of the centrifuge is 30 minutes.
Embodiment 3
[0055] The method of photoregulation and anthocyanin content measurement of pakchoi provided in this example is roughly the same as that of Example 1, the main difference being that in step S1, the soaking time in sterile water is 45 minutes, and the dark germination time is 24 hours;
[0056] In step S2, the temperature in the nursery room and the artificial climate chamber is 24°C, and the humidity is 80°C.
[0057] The photoperiod in step S2 is thirteen hours per day.
[0058] The nutrient solution prepared in step S3 is made of potassium nitrate, calcium nitrate, ammonium dihydrogen phosphate and magnesium sulfate, wherein the concentration of soluble ions is 1.2ds / m, and the pH is 5.9.
[0059] In step S5, the centrifugal temperature of the centrifuge is 4° C., the rotational speed of the centrifuge is 12000 rpm, and the separation time of the centrifuge is 25 minutes.
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