Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Ocular Surface Fluid Collection and Detection Device

A detection device and ocular surface technology, which is applied in the field of medical devices, can solve the problems of judging the patient's eye disease, the discomfort of the collected person, and the impact of the detection result, so as to avoid the influence of repeated freezing and thawing, short detection time, simple and convenient operation Effect

Active Publication Date: 2022-02-22
智德明创生物科技(无锡)有限公司
View PDF22 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In actual clinical diagnosis, it is difficult for clinicians to judge the patient's eye disease by appearance, so it is urgent to use some rapid detection technology to assist in judgment
[0004] At present, the ocular surface fluid is collected by contact, such as stimulating tear method, capillary tear collection method and adsorption tear collection method. These methods will cause discomfort to the collected subjects to varying degrees.
After collection, samples are mostly stored in cryopreservation, and rethawing samples may damage the protein during use, thus affecting the test results

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Ocular Surface Fluid Collection and Detection Device
  • Ocular Surface Fluid Collection and Detection Device
  • Ocular Surface Fluid Collection and Detection Device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] This embodiment provides an ocular surface fluid collection and detection device, including a collection part and a detection part;

[0084] Such as figure 1 with figure 2 As shown, the collection part includes a casing, a groove 1 arranged on the outer surface of the casing for collecting ocular surface fluid, and a suction hole 2 arranged at the bottom of the groove.

[0085] The groove 1 is arranged at one end of the casing, and the groove 1 forms an opening with the edge of the casing.

[0086] The groove 1 is also provided with a drainage groove 3 , one end of the drainage groove 3 is connected to the edge of the sample suction hole 2 , and the other end extends toward the opening of the groove 1 .

[0087] The outermost edge of the groove is also provided with an anti-overflow strip 4 .

[0088] Such as image 3 with Figure 4 As shown, the housing includes a card cover and a card bottom:

[0089] Both the inner surface of the card cover and the inner surfa...

Embodiment 2

[0104] This embodiment provides the method for detecting the specific IgE antibody in the ocular surface fluid sample to be tested by using the colloidal gold qualitative method in embodiment 1:

[0105] 1. Reaction principle

[0106] Anti-IgE monoclonal antibody No. 1 (T line) and goat anti-chicken polyclonal antibody (C line) are pre-immobilized on the nitrocellulose membrane of the immunochromatographic detection card, and anti-IgE monoclonal antibody 2 pre-coated with colloidal gold on the gold standard pad No. and Chicken IgY. After the collected sample of the ocular surface fluid to be tested enters the suction hole 2, the sample of the ocular surface fluid to be tested flows through the sample pad, the gold standard pad and the nitrocellulose membrane according to capillary action. When the sample contains IgE, the target object first forms a reaction complex with the colloidal gold-labeled anti-IgE monoclonal antibody No. 2, and forms a double-antibody sandwich comple...

Embodiment 3

[0125] This embodiment provides the method of detecting the specific IgE antibody in the ocular surface fluid sample to be tested by using the fluorescence quantitative method in embodiment 1:

[0126] 1. Reaction principle

[0127] Anti-IgE monoclonal antibody No. 1 (line T) and goat anti-chicken polyclonal antibody (line C) are pre-fixed on the nitrocellulose membrane, anti-IgE monoclonal antibody No. 2 and chicken polyclonal antibody pre-coated with fluorescent microspheres are pre-coated on the fluorescent pad IgY. After the collected ocular surface fluid sample to be tested enters the suction hole, the ocular surface fluid sample to be tested flows through the sample pad, the fluorescent pad and the nitrocellulose membrane according to capillary action. When the sample contains IgE, the target object first forms a reaction complex with the fluorescently labeled anti-IgE monoclonal antibody No. 2, and forms a double antibody sandwich complex with it when passing through t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a device for collecting and detecting ocular surface fluid, which relates to the technical field of medical devices. Through the design of the groove on the housing, the ocular surface fluid of the subject can be collected at the outer eyelid. Surface direct contact, to avoid eye discomfort, and the operation is simple and convenient. At the same time, the ocular surface fluid collected from the outer eyelid will slide down to the sampling hole through gravity in the groove, and then enter the matching immunochromatographic detection card, so that the relevant protein factors and pathogenic factors on the ocular surface can be detected. Immediate and rapid diagnosis of microorganisms, avoiding the influence of repeated freezing and thawing of proteins, and also has the advantages of simple operation and short detection time. The detection of ocular surface fluid related items can be completed in 15 minutes, and the results are accurate. It truly solves the on-site diagnosis and treatment of ocular surface related diseases. The problem.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a device for collecting and detecting ocular surface fluid. Background technique [0002] Immunochromatography is the pre-combination of antibodies labeled with tracer particles (colloidal gold, latex microspheres, fluorescent microspheres, etc.) The antigen or antibody pre-coated on the membrane binds to the flowing complex to develop color. This method can directly interpret the results by naked eyes, and the whole operation is simple and convenient, which is suitable for primary diagnosis and large-scale screening. [0003] The quantitative study of specific protein components in tears has been increasingly used to diagnose ocular surface diseases, judge their severity, and be used as an indication for the prevention or treatment of ocular surface diseases in clinical and scientific research. In actual clinical diagnosis, it is difficult for clinicians to judge the p...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558G01N33/53A61B10/00
CPCG01N33/558G01N33/5302A61B10/0045A61B2010/0067
Inventor 陶勇陆成慧常海涛
Owner 智德明创生物科技(无锡)有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com