Platelet preservation method

Abstract

The invention discloses a platelet preservation method. According to the invention, the influence of different temperatures on the stored platelets is discussed from the aspects of apoptosis and activation of the stored platelets, loss of physiological functions of the platelets, hemostasis thrombus formation ability of the platelets returned to the body and removal efficiency, and finally, the optimal temperature for storing the platelets is selected and is higher than 4 DEG C and not higher than 8 DEG C, preferably 6 DEG C; the platelets stored at the temperature have the best physiological function, the best thrombosis ability and the slowest in-vivo clearing efficiency. Compared with the existing accepted preservation temperature of 4 DEG C and 22 DEG C, the preservation period of the platelets is prolonged, the physiological functions of the platelets are kept to the maximum extent, meanwhile, the requirements of simple and convenient platelet storage method, long-term mass preservation, convenient transportation and the like are met, and the method is suitable for clinical general popularization and application. Meanwhile, the mechanism of apoptosis of the platelets at different preservation temperatures is also researched, and the reasons of apoptosis caused by preservation of the platelets at low temperatures (4 DEG C and 6 DEG C) and 22 DEG C are obtained.

Description

Technical field [0001] The present invention relates to a preservation method, and more particularly to a platelet preservation method. Background technique [0002] Platelet is a non-nucleated ingredient from mature mega-cytoplasm that plays a crucial effect in hemostasis and thrombosis, which is widely used to treat hemorrhagic diseases caused by platelet reduction and platelet dysfunction. And patients with bone marrow inhibitory chemotherapy. With the development of medical technology, its clinical application is increasing, but platelet products are still in a case where severe supply is short-term, and future supply and demand contradictions will be more prominent. Therefore, platelet transfusion protection is still the frontier problem of the world. Due to the short bloody life, structural and function is susceptible to a variety of factors, the preservation of platelets is urgently needed to achieve the following goals: the storage method is simple, long-term preservation...

AI Technical Summary

Problems solved by technology

[0004] Because low temperature can inhibit the growth of bacteria, some researchers have proposed low temperature storage of platelets. However, since platelets are living cells, deep low temperature (-80°C) preservation of platelets can prolong the storage period, and clinical application has proved its immediate hemostatic effect Obviously, but the disadvantages are: 1) It needs a very large and expensive cryogenic storage refrigerator (-80 ℃) or consumes a lot of liquid nitrogen; 2) It is inconvenient to carry; 3) It is difficult to transport over long distances; especially 4) After freezing Platelets can fatally impair their biological activity

There are also people trying to keep platelets at 4°C. Compared with storage at 22°C, this temperature can reduce the bacterial contamination rate of platelets and prolong the shelf life of platelets appropriately. However, platelets refrigerated at 4°C are easily activated and aggregated. It is no longer functionally active, and it is quickly phagocytosed and cleared by macrophages in the body after being injected into the human body, so that the survival period in the body is significantly shortened.

Therefore, due to the lack of necessary theoretical guidance, technical means and equipment conditions, so far, there are no conclusive and guiding research results that systematically study the influence of temperature on platelets in the world.

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