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Application of VdILV6 gene in verticillium dahliae growth and development, pathogenicity and branched chain amino acid synthesis

A technology of Verticillium dahliae and branched-chain amino acids, which can be used in applications, genetic engineering, plant genetic improvement, etc., and can solve problems such as unclear functions.

Pending Publication Date: 2021-11-26
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the function of the acetolactate synthase regulatory subunit gene (

Method used

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  • Application of VdILV6 gene in verticillium dahliae growth and development, pathogenicity and branched chain amino acid synthesis
  • Application of VdILV6 gene in verticillium dahliae growth and development, pathogenicity and branched chain amino acid synthesis
  • Application of VdILV6 gene in verticillium dahliae growth and development, pathogenicity and branched chain amino acid synthesis

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preparation example Construction

[0047] The present invention has no special limitation on the preparation method of the drug, and the preparation method of the bactericide well known in the art can be adopted. The present invention has no special limitation on the method of using the drug, and the method of using the drug well known in the art can be adopted. The medicine achieves the purpose of preventing and controlling the occurrence and development of diseases by reducing the pathogenicity of the VdILV6 gene.

[0048] Given that sulfonylurea herbicides and / or pyrimidine salicylic acid herbicides directly down-regulate VdILV6 gene, and down-regulation of VdILV6 gene can reduce the growth performance, pathogenicity and nutrient synthesis ability of Verticillium dahliae, therefore, Verticillium dahliae Application of Cladobacterium VdILV6 gene as a drug target in the control of cotton diseases.

Embodiment 1

[0051] A Construction Method of Verticillium dahliae Mutant Strain ΔVdILV6 with VdILV6 Gene Knockout

[0052] Primers were designed according to the upstream and downstream homology arms of the VdILV6 gene (nucleotide sequence shown in SEQ ID NO: 1), a knockout vector was constructed, and a mutant strain was constructed with the wild-type strain V592 as the initial strain. For details, see prior art (WANG S, XING H, HUA C, GUO H S, ZHANG J. An improved single-step cloning strategy simplifies the Agrobacterium tumefaciens-mediated transformation (ATMT)-based gene-disruption method for Verticillium dahliae. Phytopathology, 2016 , 106(6):645-652.) Obtained the VdILV6 gene knockout Verticillium dahliae mutant strain (ΔVdILV6-1, ΔVdILV6-2).

Embodiment 2

[0054] A kind of construction method of VdILV6 gene complementation mutant ECVdILV6 of Verticillium dahliae

[0055] The Verticillium dahliae mutant strain ΔVdILV6 constructed in Example 1 was used as the initial strain, see prior art (WANGS, XING H, HUA C, GUO HS, ZHANG J. An improved single-step cloning strategy simplifies the Agrobacterium tumefaciens-mediated transformation (ATMT)-basedgene-disruption method for Verticillium dahliae. Phytopathology, 2016, 106(6):645-652.) Obtained the complementation mutants of Verticillium dahliae VdILV6 gene (ECVdILV6-1 and ΔVdILV6-2).

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Abstract

The invention provides application of an acetolactate synthase (AHAS) regulation subunit gene VdILV6 in growth and development, pathogenicity and branched chain amino acid synthesis of verticillium dahliae, and belongs to the technical field of functional gene detection. According to the invention, a knockout mutant delta VdILV6 and a complementary mutant are taken as experimental subjects, and the effects of the VdILV6 are respectively analyzed from the aspects of growth and development, pathogenicity and branched chain amino acid synthesis. The result shows that the growth speed of the delta VdILV6 bacterial colony is reduced, and more aerial hyphae are generated; microsclerotia is not formed; the sporulation quantity is obviously reduced; the pathogenicity is obviously reduced; VdILV6 is an essential gene for synthesis of branched-chain amino acids Val and Ile, and Leu has a feedback inhibition effect on synthesis of branched-chain amino acids. AHAS is an action target of a herbicide, and VdILV6 can be applied to prevention and treatment of cotton verticillium wilt. The invention has important value for prevention and control of verticillium dahliae.

Description

technical field [0001] The invention belongs to the technical field of functional gene detection, and specifically relates to the application of VdILV6 gene in the growth and development, pathogenicity and branched-chain amino acid synthesis of Verticillium dahliae. Background technique [0002] Verticillium dahliae (Verticillium dahliae) belongs to the fungus of the genus Verticillium of the subphylum Ignomorum. Verticillium dahliae has a very wide range of hosts. According to foreign reports, it can harm 660 species of plants in 38 families, including 184 species of crops and 153 species of weeds. my country has identified at least 80 species of host plants in 20 families. Field crops include sunflower, eggplant, pepper, tomato, tobacco, potato, melon, watermelon, cucumber, peanut, kidney bean, mung bean, soybean, sesame, sugar beet, etc., and general gramineous crops Such as rice, wheat, corn, millet, sorghum, etc. are not affected. Verticillium dahliae is the main patho...

Claims

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Application Information

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IPC IPC(8): C12N15/54A01N47/36A01N43/54A01P13/00
CPCC12N9/1022C12Y202/01006A01N47/36A01N43/54
Inventor 黄家风邵胜楠都业娟
Owner SHIHEZI UNIVERSITY
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