Liquid strain clinker bag type strain cultivation method for agaricus bisporus

Abstract

The invention relates to a liquid strain clinker bag type strain cultivation method for agaricus bisporus. The method specifically comprises the following steps: (1) liquid strain preparation: (1.1) liquid shake flask strain preparation; and (1.2) fermentation tank liquid strain preparation; (2) strain bag preparation; and (3) fruiting production management. A special culture medium formula is adopted for liquid strains, the PH value can reach 8.5 or above to achieve a weak base environment, and the liquid strains can be completely germinated and matured after being butted with strain bags. The liquid strains are good in strain age activity, the first-stage strain culture only needs 6 days, the second-stage strain culture only needs 7 days, the culture time is greatly shortened, the culture efficiency is improved, and the liquid strains are convenient for automatic mechanical seeding. The liquid strains are cultured in a fermentation tank, and are subjected to 121 DEG C high-pressure sterilization after being butted with the strain bags, so that viruses, germs and worm eggs in the liquid strains can be completely killed, a sterile state is achieved, the quality of the strains is improved, and the safety guarantee of pure green organic food without bacteria, viruses, bacilli and the like is further achieved.

Description

technical field [0001] The invention relates to the technical field of Agaricus bisporus cultivation, in particular to a clinker bag type culture method for Agaricus bisporus liquid bacteria. Background technique [0002] Agaricus bisporus is also known as white mushroom, mushroom, and mushroom. Agaricus bisporus is a mushroom that is cultivated and consumed around the world. It is known as the "World Mushroom" and can be sold fresh, canned, or salted. The mycelium of Agaricus bisporus is also used as a raw material for medicine. China's Agaricus bisporus is most cultivated in Fujian, Shandong, Henan, Zhejiang and other provinces. Cultivation methods include mushroom house cultivation, greenhouse frame cultivation and greenhouse border cultivation. Different regions, different climatic conditions and different seasons can adopt suitable cultivation methods. It is widely distributed and widely cultivated in China. However, with the continuous development of Agaricus bispo...

AI Technical Summary

Problems solved by technology

The cultivation of the first-level and second-level strains of the solid dung soil fermentation and the Dutch tunnel fermentation are all cultured by wheat grain strains, and the cultivation time of the first-level strains and the second-level strains are both 30-35 days, and the production cycle is relatively It is long, and the cultivation of solid strains uses dung grass fermentation material. The production of fermentation material will produce a large amount of waste water and waste gas to pollute the environment, and the dung grass fermentation strains are conventionally pasteurized after making the bacteria bag, there will be Viruses, germs, and insect eggs remain, which cannot be completely removed, causing some viruses and insect eggs to remain in the mushroom

[0004] At present, the cultivation techniques of liquid strains have been disclosed in the cultivation of other mushrooms at home and abroad, but they are all aimed at acidic or weakly acidic mushrooms, and Agaricus bisporus is a special weakly alkaline mushroom. The currently disclosed liquid strains The technology cannot prepare alkaline strains, which cannot be inoculated with Agaricus bisporus for cultivation