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Ultrasmall-particle-size nuclear magnetic resonance contrast agent as well as preparation method and application thereof

A nuclear magnetic resonance and contrast agent technology, applied in the fields of biology, chemistry and medical material science, can solve the problems of harsh reaction conditions, and achieve the effects of mild reaction conditions, high contrast performance, and low environmental pollution

Inactive Publication Date: 2021-11-02
WUHAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, it can not only provide the biocompatible contrast agent pursued in the field of clinical medical imaging, but also solve the problems of harsh reaction conditions in traditional synthesis methods.

Method used

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  • Ultrasmall-particle-size nuclear magnetic resonance contrast agent as well as preparation method and application thereof
  • Ultrasmall-particle-size nuclear magnetic resonance contrast agent as well as preparation method and application thereof
  • Ultrasmall-particle-size nuclear magnetic resonance contrast agent as well as preparation method and application thereof

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Embodiment 1

[0034] 1. Preparation of MnSe nanocrystals:

[0035] S.1) Inoculate Staphylococcus aureus strains on LB solid medium by streaking method, and incubate in a constant temperature shaker (37° C.) for 20 h to obtain Staphylococcus aureus colonies;

[0036] S.2) Transfer the single colony in step S.1) to an Erlenmeyer flask (volume 40mL) filled with 10mL LB liquid medium, and incubate in a constant temperature shaker (37°C, 180rpm) for 12h until the cells recover vitality;

[0037] S.3) Inoculate the activated bacterial liquid obtained in step S.2) into an Erlenmeyer flask (volume 250 mL) containing 100 mL of LB liquid medium at an inoculation ratio of 1:100, and incubate for 12 hours until the bacterial growth is stationary;

[0038] S.4) Use the bacterial solution obtained in step S.3) as a biosynthesizer, add sodium selenite solution (final concentration 5 mmol / L) to it, and incubate for 9 hours on a constant temperature shaker (37°C, 180rpm) to obtain intracellular "Selenium-...

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Abstract

The invention discloses an ultrasmall-particle-size nuclear magnetic resonance contrast agent as well as a preparation method and application thereof. The contrast agent is a MnSe nanocrystal. According to the preparation method of the contrast agent, staphylococcus aureus is used as a biological synthesizer, sodium selenite is reduced to obtain low-valence Se through the selenium-rich metabolism process of cells, the low-valence Se reacts with a manganese precursor Mn < 2 + > in the cells, and the MnSe nanocrystal which is good in monodispersity, small and uniform in particle size and high in longitudinal relaxation rate under the water phase condition can be obtained. The ultrasmall-particle-size nuclear magnetic resonance contrast agent provided by the invention has good biocompatibility and high contrast performance, can be passively gathered to a tumor part through high permeability and retention effect of a solid tumor, realizes high-contrast nuclear magnetic resonance imaging, and can be applied to living tumor imaging. In addition, the preparation method is simple and convenient, does not involve high-temperature, high-pressure and toxic solvents, is safe and green, and can be widely applied to the fields of chemistry and material science.

Description

technical field [0001] The invention relates to the fields of biology, chemistry and medical material science, in particular to a nuclear magnetic resonance contrast agent with an ultra-small particle size and a preparation method and application thereof. Background technique [0002] Magnetic resonance imaging (MRI) is widely used in the field of clinical medical imaging due to its advantages of non-invasive diagnosis, no ionizing radiation process, and ability to provide high-resolution image information. Magnetic resonance imaging technology can provide anatomical imaging of human tissues because water molecules in different tissues of the human body have different relaxation times under the action of an external magnetic field. However, there is not much difference in the relaxation time of water molecules between early diseased tissue and normal tissue. Therefore, in order to obtain medical images that can accurately reflect lesion information, the help of MRI contrast ...

Claims

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Application Information

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IPC IPC(8): A61K49/06A61K49/18B82Y5/00B82Y15/00C12N1/20C12P3/00C12R1/445
CPCA61K49/06A61K49/1827B82Y5/00B82Y15/00C12N1/20C12P3/00
Inventor 崔然陈诗卉张志凌
Owner WUHAN UNIV
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