Process for preparing 7-ADCA by taking DAOC fermentation liquor as raw material

Abstract

The invention relates to a method for separation and purification of DAOC and preparation of 7-ADCA. The method comprises the following steps: (1) pretreatment of DAOC fermentation liquor: firstly, adjusting the pH value of the fermentation liquor, and conducting filtering via a filter screen to remove insoluble large-particle impurities; (2) primary extraction of treatment liquor via two macroporous adsorption resin tandem columns; (3) decoloration treatment of desorption liquid via anion exchange resin; (4) conversion into 7-ADCA via one-step acylase lysis; (5) decolorization with activated carbon; and (6) pH value adjustment and crystallizing. According to the invention, the use amount of an organic solvent can be reduced, and environment protection is facilitated. According to the invention, the production of pollutants can be reduced in virtue of regeneration and recycling of the resin, the process is simple to operate, the disadvantages of conventionally used strong oxidant are changed, high safety is obtained, the extraction rate of the DAOC fermentation liquor is high and can achieve 95% or more, the total yield of a 7-ADCA product is 46-48%, the purity of the obtained 7-ADCA product is high and can achieve 99.5 or more, and the content of 7-ADCA can achieve 99% or more.

Description

technical field [0001] The invention belongs to the technical field of separation and extraction of biologically active components and preparation of a compound, and more specifically relates to a method for extracting and separating DAOC from biological fermentation liquid and preparing the compound 7-ADCA. Background technique [0002] 7-aminodeacetoxycephalosporanic acid (7-ADCA) is the main starting material for the synthesis of semi-synthetic antibiotics cephalosporins, such as cefadroxil, cephalexin and cephradine. Its molecular structure is as follows: [0003] [0004] 7-Aminodeacetoxycephalosporanic acid (7-ADCA) is a substrate used in the preparation of specific cephalosporins with functional deletions at position 3. At present, its domestic preparation method is mainly chemical method, patent CN101735246 and literature (Liu Dongzhi, Qu Hongmei, Zhang Tianyong. Synthesis progress of 7-ADCA [J] Foreign Medicine and Antibiotics Volume, 1998, 19(4): 267-268 .) di...

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Problems solved by technology

Its patent No. CN1357051A of Leon Company of Spain discloses a production process of utilizing biological method to prepare 7-ADCA, and introduces the process of using DAOC as substrate to produce 7-ADCA, but in the preparation process, DAOC needs to be concentrated first, and then , using organic solvents for crystallization to obtain low-purity DAOC crystals, and then using two-step enzymatic methods combined with strong oxidants for cracking, and finally using organic matter for separation and purification. Although this process simplifies the original production process, but in production, The process is still relatively complicated, and organic solvents and strong oxidants are still needed, and the purity and yield of the product are relatively low

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