Split nucleic acid aptamer for specifically detecting ciprofloxacin and application of split nucleic acid aptamer

A technology of nucleic acid aptamer and ciprofloxacin, which is applied in the field of detection of fluoroquinolone antibiotics, can solve the problems of expensive detection instruments, inconvenient carrying, and complicated operation, and achieve rapid detection, high sensitivity, and accurate experimental results Effect

Active Publication Date: 2021-09-24
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although these methods can accurately detect the content and type of antibiotics, these methods generally have problems such as time-consuming, complicated operation, expensive detection equipment, inconvenient carrying, and the need for professional technicians.

Method used

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  • Split nucleic acid aptamer for specifically detecting ciprofloxacin and application of split nucleic acid aptamer
  • Split nucleic acid aptamer for specifically detecting ciprofloxacin and application of split nucleic acid aptamer
  • Split nucleic acid aptamer for specifically detecting ciprofloxacin and application of split nucleic acid aptamer

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Embodiment 1

[0027] 1. Aptamer Design

[0028] Split-type nucleic acid aptamer is to break the original complete nucleic acid aptamer into two or more fragments at the proper position. These fragments can specifically recognize the target and form a complex with the target when the target exists. , so that the aptamer fragments are close to each other, thereby stimulating the generation of detection signals. The complete nucleic acid aptamer chain of CIP used in this example is composed of 98-mer DNA sequence. In order to effectively reduce the length of the DNA probe sequence, thereby reducing the synthesis cost and improving the purification rate of the fluorescein-modified nucleic acid aptamer fragment, more importantly, it can effectively avoid the defect that the long-sequence DNA probe is easy to form a secondary structure. To facilitate the formation of the recognition configuration, in this embodiment, a segment of the cleavage-type nucleic acid aptamer is designed with a length o...

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PUM

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Abstract

The invention discloses a nucleic acid aptamer for specifically detecting ciprofloxacin and application of the nucleic acid aptamer, and belongs to the field of detection of fluoroquinolone antibiotics. The invention provides two nucleic acid sequences, one of which is as shown in SEQ ID NO.1, and the other one of which is as shown in SEQ ID NO.2. A detection experiment result on ciprofloxacin in a buffer solution after FAM is modified at a 5'-end of the segment with a nucleotide sequence as shown in SEQ ID NO.1 shows that the nucleic acid aptamer provided by the invention is high in combination specificity with ciprofloxacin and is slightly interfered by other fluoroquinolone antibiotics, and the lowest detection limit is as low as 5nM; And the method has the advantages of low requirements on detection environment and time, simplicity in operation and rapidness in detection.

Description

technical field [0001] The invention belongs to the detection field of fluoroquinolone antibiotics, and more specifically relates to a split-type nucleic acid aptamer for specific detection of ciprofloxacin and its application. Background technique [0002] Since Italian scientist Bartolomeo Gosio isolated the first antibiotic - mycophenolic acid (MPA) from Penicillium, humans have developed thousands of antibiotics, which are widely used in medicine, animal husbandry and aquaculture, and agriculture. prevention and other fields. Antibiotics can be divided into fluoroquinolones, tetracyclines, penicillins, macrolides, aminoglycosides, β-lactams, sulfonamides, etc. according to their chemical structure or mechanism of action, among which fluoroquinolones, macrolides Esters and aminoglycosides are mostly used in the treatment of human clinical diseases, while penicillins, tetracyclines and macrolides are widely used in veterinary medicine. In addition, various antibiotics are...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115G01N33/53
CPCC12N15/115G01N33/5308C12N2310/16
Inventor 李胎花王婷吕新月宫磊金龙
Owner NANJING FORESTRY UNIV
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