A cell capture and detection micro-fluidic chip

Abstract

The invention discloses a cell capture and detection micro-fluidic chip, which comprises a flow channel substrate and an electrode substrate,. The flow channel substrate is provided with a micro-flow channel structure, and the micro-flow channel structure comprises a sample inlet, a detection micro-cavity and a sample outlet. The sample inlet is jointly formed by a cell injection port and a buffer solution injection port which are respectively used for enabling a cell solution and a phosphate buffer salt solution to flow in, the two injection ports are converged into the same flow channel, and the tail end of the flow channel is gradually expanded and converged into an open cavity, namely a detection microcavity; the detection microcavity is enclosed by four hyperbolic curves, four hyperbolic curve type electrodes are arranged on the wall surface of the cavity, a special alternating electric field can be generated in the microcavity to realize cell capture and cell detection, and an inlet and three outlets are formed in four corners of the cavity; the sample outlet comprises two lateral outlets and an opposite outlet, the lateral outlets are used for outflow of non-captured cells, and the opposite outlet is used for regulating and controlling the state of a flow field. According to the cell capture and detection micro-fluidic chip, capture and dielectric detection of specific types of cells can be realized.

Description

technical field [0001] The invention relates to the field of microfluidic technology, in particular to a cell capture and detection microfluidic chip. Background technique [0002] Microfluidics is the precise prediction, control, and manipulation of the behavior of small-scale fluids (usually below the submillimeter). It is an emerging technology involving multidisciplinary fields, suitable for the transmission, mixing and detection of trace liquids. Since the fluid at the submillimeter scale is mainly dominated by surface forces, and its internal motion is dominated by peristaltic flow, the substances in the sample solution can be precisely controlled by the outside world, thereby achieving precise sample manipulation. This advantage makes microfluidic technology widely used in the field of biological research, such as DNA chip detection, organoid culture, fluorescent flow cytometry screening, etc. For cell screening and sorting by physical means, microfluidic technology...

AI Technical Summary

Problems solved by technology

However, the above-mentioned technologies will seriously affect the biological functions and biological characteristics of cells, making it impossible for researchers to carry out subsequent biological research, and greatly restricting clinical treatment methods and medications.

Existing dielectrophoresis patented technologies such as patent CN 106244416 A are limited to the control of particles by electric field, and the design of flow field is relatively simple, ignoring the influence of flow field on particle movement, and lacking effective methods for particle capture

Images