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Chicken feather color character related gene MC1R molecular marker and application thereof

A molecular marker and feather color technology, applied in DNA/RNA fragmentation, microbial determination/inspection, recombinant DNA technology, etc., can solve the problem of chicken feather color traits molecular marker-assisted selection has not been used, and the MC1R gene chicken feather color has not been established. Problems such as detection methods of molecular markers of traits

Pending Publication Date: 2021-08-27
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] However, at present, from a genetic point of view, there is no established detection method for the MC1R gene as a molecular marker for chicken feather color traits, and at the same time, there is no application of this gene as a molecular marker for chicken feather color traits.

Method used

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  • Chicken feather color character related gene MC1R molecular marker and application thereof
  • Chicken feather color character related gene MC1R molecular marker and application thereof
  • Chicken feather color character related gene MC1R molecular marker and application thereof

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Embodiment 1

[0029] Establishment of a molecular detection method for chicken black feather and hemp feather feather color traits

[0030] (1) Primers:

[0031]Using the DNA sequence of the chicken MC1R gene (the reference sequence GeneBank accession number is: D78272.1) as a template, a pair of primers MC1R-2F / MC1R-2R were designed to obtain the DNA sequence shown in the sequence SEQ ID NO.1. The sequence is completely The length is 1308bp ( TCACCATCCTGCTGGGAGTCTTCTTCATCTGCTGGGGGCCCTTCTTCTTCCACCTCATCCTCATCGTCACCTGCCCCACCAACCCCTTCTGCACCTGCTTCTTCAGCTATTTCAACCTCTTCCTCATCCTCATCATCTGCAATTCAGTGGTCGATCCCCTGATCTATGCCTTCCGGAGCCAGGAGCTCCGGCGGACGCTGCGGGAGGTGGTGCTGTGCTCCTGGTAGGAGGCGGCACAGACAGGAGGATGGATGGATGGATGGATGGACGGATGGACGGATGGATGGATGGACAAACAGATGGGTGGATGGACAGATGGGTGGATGGACAAACAGACGCACCGCGGGGTGTCCCCTGGGTGCCCCAGTGCAGCTGGGGTTGGGCTGCCTGGCCTCGCGCTCCCAAATAAAGG)。 The primer sequences used are respectively shown in SEQ ID NO.2 and SEQ ID NO.3, specifically as follows:

[0032] Forward primer MC1R-2F: 5...

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Abstract

The invention discloses a chicken feather color character related gene MC1R molecular marker and application thereof, and relates to the technical field of chicken marker-assisted selection. Compared with the DNA sequence of the coding region of the MC1R gene, the molecular marker comprises SNP1 to SNP5. The feather color characters of different types of black feathers and hemp feathers are selected, the feather color selection breeding process is accelerated, the feather color consistency of chicken flocks is improved, and when SNP1-SNP5 is CTGAC, the proportion of the breast black feather type and the breast back black * black hemp feather color phenotype of F2-generation cocks is increased; when the SNP1 to SNP5 are CTGAG, the ratio of the yellow feather type of the breast of the F2-generation hens to the phenotype of yellow x jute feather color of the breast and the back of the F2-generation hens is increased; and when the SNP1 to SNP5 are TCACC, the ratio of the phenotypes of the mayplume the mayplume and the mayplume color of the chest of the F2-generation hens to the phenotypes of the red mayplume and the red and the mayplume color of the chest of the F2-generation cocks is increased.

Description

technical field [0001] The invention relates to the technical field of marker-assisted selection of chickens, in particular to a molecular marker of chicken feather color trait related gene MC1R and its application. Background technique [0002] Our country is rich in local chicken breed resources. Local chicken breeds are popular with consumers because of their delicious meat quality. They have great potential as breeding materials. However, due to the uneven feather color within the breed, the genetic law and mechanism of feather color have not been studied in depth. Factors such as these will greatly affect the utilization of fine varieties and the economic benefits of their commodities. [0003] For a long time, the feather color of birds and the coat color and skin color of mammals have been the focus of pigment research. Feather color can serve as a genetic marker that helps identify breeds, populations and breeding groups based on their characteristics. The use of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/124C12Q2600/156C12Q2600/172
Inventor 王艳吴日富瞿浩舒鼎铭刘天飞罗成龙计坚
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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