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Fusion protein of midbrain astrocyte-derived neurotrophic factor for preventing and treating obesity

A technology of astrocytes and neurotrophic factors, applied in the field of biomedicine, can solve the problems of inconvenient clinical use and short plasma half-life, and achieve the effect of prolonging the half-life in vivo and broad application prospects

Active Publication Date: 2021-08-24
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, it is well known that protein drugs are easily degraded by proteases in the gastrointestinal tract and are limited to injection administration. Moreover, due to the short plasma half-life of protein peptide drugs, in order to achieve effective therapeutic concentrations, repeated injections are often required every day. Bring a lot of inconvenience to clinical use

Method used

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  • Fusion protein of midbrain astrocyte-derived neurotrophic factor for preventing and treating obesity
  • Fusion protein of midbrain astrocyte-derived neurotrophic factor for preventing and treating obesity
  • Fusion protein of midbrain astrocyte-derived neurotrophic factor for preventing and treating obesity

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Embodiment 1

[0051] Example 1 Preparation of fusion protein MANF-Fc of the present invention

[0052] Whole gene synthesis MANF-Fc gene, its nucleotide sequence (SEQ ID No.5) is as follows:

[0053]

[0054]

[0055] GAATTC 1-6 of the above sequence is the restriction site EcoR I, CCGCCGCCACC 7-17 is the KOZAK sequence, TGATAA 1257-1262 is the stop codon, and AAGCTT 1261-1266 is the restriction site HindIII.

[0056] Then the MANF-Fc gene was inserted into the expression vector proEM by double enzyme digestion, and the accuracy of the final expression vector was confirmed by enzyme digestion and sequencing.

[0057] Next, the MANF-Fc plasmid was transferred to the DH5α clone strain, and the specific operations were as follows:

[0058] 1. Take 80-100ng of expression plasmid containing MANF-Fc gene and add it into the prepared DH5α competent cells.

[0059] 2. Transform the competent cells after adding the plasmid through heat shock.

[0060] 3. Add the transformed competent cells in...

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Abstract

The invention relates to a fusion protein of a midbrain astrocyte-derived neurotrophic factor for preventing and treating obesity, and belongs to the technical field of biological medicines. The invention provides a fusion protein of a midbrain astrocyte-derived neurotrophic factor. The fusion protein at least contains the following two fragments: an amino acid sequence of the midbrain astrocyte-derived neurotrophic factor and an amino acid sequence of an immune globulin Fc fragment. The fusion protein is good in stability and long in half-life period, can be used for treating obesity, diabetes mellitus and other metabolic diseases, and has a good application prospect.

Description

technical field [0001] The invention relates to a fusion protein of midbrain astrocyte-derived neurotrophic factor for preventing and treating obesity, and belongs to the technical field of biomedicine. Background technique [0002] With the development of social economy and the change of people's lifestyle, obesity has become a serious global public health problem, and it is an important risk factor for the occurrence and development of hypertension, diabetes, and cardiovascular and cerebrovascular diseases. Searching for safe and effective ways to reduce fat and lose weight is one of the hotspots in current medical research. [0003] The main cause of obesity is the long-term abnormal regulation of energy balance, including excessive energy intake and insufficient energy consumption, resulting in excess energy being stored in adipose tissue in the form of triglycerides. Adipose tissue can be divided into white adipose tissue, brown adipose tissue and beige adipose tissue....

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A23L33/10A61K38/18A61K47/64A61P3/04A61P3/10A61P5/50
CPCC07K14/475A23L33/17A61P3/04A61P3/10A61P5/50C07K2319/30A23V2002/00A61K38/00A23V2200/332A23V2200/328A23V2250/542
Inventor 何金汗李艳萍吴桐
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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