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Colon-targeted oral probiotic microcapsule and preparation method and application thereof

A technology of colon targeting and probiotics, which is applied in the field of biomedical engineering, can solve the problems of unfavorable colonization of probiotics and limited protection ability of probiotics, achieve excellent adhesion-promoting performance, ensure biological safety, and promote colonization effect

Active Publication Date: 2021-08-20
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the materials that are limited by this method have extremely limited protective ability for probiotics and the ambiguous release site of probiotics is not conducive to the effective colonization of probiotics in the colon

Method used

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  • Colon-targeted oral probiotic microcapsule and preparation method and application thereof
  • Colon-targeted oral probiotic microcapsule and preparation method and application thereof
  • Colon-targeted oral probiotic microcapsule and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Dissolve 5.0 g of cornstarch in NaOH solution with a mass fraction of 15%, stir magnetically at 60° C. for 2 h, cool, wash, and dry for later use. Take 2g of pretreated cornstarch into 50mL Na 2 CO 3 / NaHCO 3 (pH=10.83) buffer solution, stir to dissolve. Add 40mg TEMPO and 0.6mg NaBr successively, and continue stirring until completely dissolved. Finally, 8 mL of NaClO (pH=10) solution was added and reacted for 5 h. Add excess ethanol to precipitate the oxidized cornstarch, and dry it under vacuum at 60°C for later use.

[0045] Take 0.5 g of the spare oxidized corn starch mentioned above, dissolve it in 50 mL of deionized water, and fill it with nitrogen to remove oxygen for 30 min. Then in N 2 Under the protection of , add EDC and NHS (EDC: NHS: oxidized cornstarch = mass ratio is 10:10:1), the mass ratio is 10:10:1, activate 1h. Then slowly add 2% (w / v) chitosan acetic acid solution (molecular weight: 1000, deacetylation degree: 70%), and stir for 24 hours. ...

Embodiment 2

[0047] Dissolve 5.0 g of wheat starch in NaOH solution with a mass fraction of 15%, stir magnetically at 60° C. for 2 h, cool, wash, and dry for later use. Take 2g of pretreated wheat starch into 50mL Na 2 CO 3 / NaHCO 3 (pH=10.83) buffer solution, stir to dissolve. Add 40mg TEMPO and 0.6mg NaBr successively, and continue stirring until completely dissolved. Finally, 8 mL of NaClO (pH=10) solution was added and reacted for 5 h. Add excess ethanol to precipitate the oxidized wheat starch, and dry it under vacuum at 60°C for later use.

[0048] Take 0.5 g of the spare oxidized wheat starch mentioned above, dissolve it in 50 mL of deionized water, and fill it with nitrogen to remove oxygen for 30 min. Then in N 2 Under the protection of , add EDC and NHS (EDC: NHS: oxidized cornstarch = mass ratio is 10:10:1), the mass ratio is 10:10:1, activate 1h. Then slowly add 2% (w / v) chitosan acetic acid solution (molecular weight: 100,000, degree of deacetylation: 95%), and stir fo...

Embodiment 3

[0050] Dissolve 5.0 g of potato starch in NaOH solution with a mass fraction of 15%, stir magnetically at 60° C. for 2 h, cool, wash, and dry for later use. Put 2g of pretreated potato starch into 50mL Na 2 CO 3 / NaHCO 3 (pH=10.83) buffer solution, stir to dissolve. Add 40mg TEMPO and 0.6mg NaBr successively, and continue stirring until completely dissolved. Finally, 8mLNaClO (pH=10) solution was added and reacted for 5h. Add excess ethanol to precipitate the oxidized potato starch, and dry it under vacuum at 60°C for later use.

[0051] Take 0.5 g of the spare oxidized potato starch mentioned above, dissolve it in 50 mL of deionized water, and fill it with nitrogen to remove oxygen for 30 min. Then in N 2 Under the protection of , add EDC and NHS (EDC: NHS: oxidized cornstarch = mass ratio is 10:10:1), the mass ratio is 10:10:1, activate 1h. Then slowly add 2% (w / v) chitosan acetic acid solution (molecular weight: 50,000, deacetylation degree: 80%), and stir for 24 ho...

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Abstract

The invention discloses a colon-targeted oral probiotic microcapsule and a preparation method and application thereof, and belongs to the field of biomedical engineering. A core of the microcapsule is a probiotics-containing calcium alginate gel core, and a shell of the microcapsule is coated with multiple layers of chitosan-starch. The microcapsule has a compact coating structure, can prevent probiotics from being eroded by gastric acid and cholate and can be triggered by trypsin, so that a carrier is efficiently disintegrated in a fixed point at the colon, and a targeting property is excellent; a chitosan coating on the periphery of the carrier endows the probiotics with relatively high intestinal mucosa adhesion; and oligosaccharides generated after starch on the periphery of the carrier is digested can serve as prebiotics substances to promote growth of the probiotics after fixed-point adhesion. Therefore, the oral probiotic micro-capsule provided by the invention is an oral probiotic micro-capsule that is resistant to both the gastric acid and the cholate, has a clear release site and high release degree, improves thallus adhesion and promotes the growth of the probiotics.

Description

technical field [0001] The invention belongs to the field of biomedical engineering, and in particular relates to a colon-targeted oral probiotic microcapsule and a preparation method and application thereof. Background technique [0002] The gut microbiota has recently received a lot of attention as one of the most promising targets. Supplementing probiotics can improve the distribution of intestinal microbial flora, which has significant significance for the treatment of clinical diseases such as diabetes, cardiovascular disease, liver disease, and tumors. At present, fecal microbiota transplantation (FMT) therapy has been used clinically to show good results in the treatment of inflammatory bowel disease, Clostridium difficile infection and other diseases. At present, there are two main methods of FMT drug delivery: oral delivery and endoscopic implantation. [0003] Oral administration of probiotic microcapsules is convenient, well tolerated by patients, and easier to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/36A61K35/741A61K47/61A61P1/00A61P29/00
CPCA61K9/5036A61K47/61A61K35/741A61K9/5073A61K9/0053A61P1/00A61P29/00
Inventor 马栋程启坤程兆怡
Owner JINAN UNIVERSITY
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