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Human growth differentiation factor-15 magnetic particle chemiluminescence detection kit and application thereof

A technology for detection of growth differentiation factors and chemiluminescence, which is applied in the field of medicine, can solve the problems of ELISA detection method, such as long time-consuming process, inability to realize rapid and large-scale detection, and many manual operations, to achieve improved sensitivity, small magnetic particles, and improved sensitivity Effect

Pending Publication Date: 2021-08-13
北京赛诺浦生物技术有限公司 +1
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Problems solved by technology

[0006] The ELISA detection method is time-consuming, requires many manual operations, has low detection sensitivity and poor repeatability, and is not suitable for rapid diagnosis of acute myocardial patients in clinical practice.
The test strip method needs to be equipped with a special instrument, which is used in conjunction with the detection test strip. The detection sensitivity is low, the detection throughput is small, and it cannot achieve rapid and large-scale detection.

Method used

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  • Human growth differentiation factor-15 magnetic particle chemiluminescence detection kit and application thereof
  • Human growth differentiation factor-15 magnetic particle chemiluminescence detection kit and application thereof
  • Human growth differentiation factor-15 magnetic particle chemiluminescence detection kit and application thereof

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Embodiment 1

[0073] Example 1. Preparation of magnetic particle chemiluminescence detection kit for detection of human growth differentiation factor-15 and establishment of detection system

[0074] 1. Experimental materials: mouse anti-human GDF-15 monoclonal antibody [23G10.F8], [7C12.B3.F2] (purchased from ABCAM Company, article number: ab105732, ab106112), recombinant human GDF-15 antigen (purchased from ABCAM company, catalog number: ab50077).

[0075] 2. Preparation of FITC-labeled GDF15 monoclonal antibody 1

[0076] Main materials: fluorescein 5(6)-isothiocyanate (FITC); GDF15 monoclonal antibody 1 [23G10.F8]; sodium bicarbonate.

[0077] Preparation Process:

[0078] (1) Elute through the PD-10 column, replace the buffer of GDF15 monoclonal antibody 1 with 200mmol / L sodium bicarbonate buffer at pH9.0, and dilute the antibody to 3mg / ml.

[0079] (2) Put the antibody into a dialysis bag, put it into a pH 9.0 sodium bicarbonate buffer solution containing 0.5 mg / mL FITC, and react ...

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Abstract

The invention discloses a human growth differentiation factor-15 magnetic particle chemiluminescence detection kit and application thereof, the kit contains a liquid reagent R1 of a GDF15 monoclonal antibody 1 marked with FITC, a liquid reagent R2 of a GDF15 monoclonal antibody 2 marked with ALP, a magnetic particle reagent R3 coupled with an anti-FITC antibody, a calibration product and a quality control product, the GDF15 monoclonal antibody 1 and the GDF15 monoclonal antibody 2 are monoclonal antibodies with different antigen recognition epitopes, and the GDF15 monoclonal antibody 1 and the GDF15 monoclonal antibody 2 and a growth differentiation factor-15 can form a compound in an antibody-antigen-antibody form. The GDF15 is detected through a magnetic particle chemiluminescence method, the method is high in detection speed, high in sensitivity, large in detection flux and easy to operate in clinical detection, full-automatic operation can be achieved on a luminescence instrument, time and labor are saved, and convenience and rapidness are achieved.

Description

technical field [0001] The invention relates to a magnetic particle chemiluminescence detection kit and its application, in particular to a magnetic particle chemiluminescence detection kit for detecting human growth differentiation factor-15 and its application. The invention belongs to the technical field of medicine. Background technique [0002] Growth differentiation factor-15 (growth differentiation factor-15, GDF15) belongs to the growth differentiation factor (GDFs) family and is a member of the transforming growth factor-β (transforming growth factor B, TGF-B) superfamily. Studies have shown that the human GDF15 gene sequence is located at 19p13.1-13.2, contains 924 base pairs, consists of 2 exons and 1 intron, and is separated by its intron. The gene of GDF15 is first transcribed and translated into a polypeptide of 308 amino acid residues, and finally a mature protein of GDF15 comprising 224 amino acid residues is formed. [0003] Under normal circumstances, GDF...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/543G01N33/535G01N33/533G01N21/76
CPCG01N33/74G01N33/577G01N33/54326G01N33/535G01N33/533G01N21/76G01N2333/475
Inventor 李君丽朱海燕周金池王兰珍
Owner 北京赛诺浦生物技术有限公司
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