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A microfluidic chip for nucleic acid extraction and PCR detection and its application

A microfluidic chip and nucleic acid extraction technology, which is applied to specific-purpose bioreactors/fermenters, fluid controllers, and laboratory containers, etc., which can solve inconvenient operation, slow sample change speed, and low analysis throughput and other issues to achieve the effect of avoiding secondary pollution

Active Publication Date: 2022-07-19
CHINESE ACAD OF INSPECTION & QUARANTINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The common feature is that the sample change operation requires three-dimensional movement of the probe or the sample plate. The disadvantages are that the three-dimensional control mechanism is complicated, large in size, high in cost, slow in sample change speed, and low in analysis throughput. It cannot be completed through nucleic acid extraction and purification and PCR amplification, it needs to be completed separately, and the operation is inconvenient

Method used

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  • A microfluidic chip for nucleic acid extraction and PCR detection and its application
  • A microfluidic chip for nucleic acid extraction and PCR detection and its application

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Effect test

Embodiment 1

[0027] A microfluidic chip for nucleic acid extraction and PCR detection, including nucleic acid extraction reaction chamber, purification reaction chamber and PCR reaction chamber, these three reaction chambers can be designed on the same chip, such as figure 1 As shown, the nucleic acid extraction reaction chamber 1 and the purification reaction chamber 2 are connected to the liquid inlet chamber with two branches at their front ends, the liquid inlet chamber is connected with the capillary for buffering when the solution enters, and the rear end is connected to a liquid intake chamber, The liquid taking chamber is connected with the capillary tube, and the solution in the reaction chamber is sucked out by a suction pump. The front end of the PCR reaction chamber is connected to a three-branch liquid inlet chamber. Take the liquid tank. Nucleic acid extraction reaction chamber 1 is a reaction solution for adding magnetic beads to extract nucleic acid, which is a reaction cha...

Embodiment 2

[0030] In the microfluidic chip of this embodiment, two chips are arranged for nucleic acid extraction and PCR detection. One chip is provided with a nucleic acid extraction reaction chamber and a purification reaction chamber, and the other chip is provided with one or more PCR reaction chambers. warehouse. Other settings are required by example.

[0031] The specific reaction process of the microfluidic chip of this embodiment is as follows: firstly, the cells are lysed outside the chip to obtain the lysed sample liquid. The sample liquid enters the liquid inlet chamber connected to the nucleic acid extraction reaction chamber through the capillary, the pre-embedded magnetic beads are added to the nucleic acid extraction reaction chamber in advance, and the lysed sample liquid in the liquid inlet chamber is added to the nucleic acid extraction reaction with pre-embedded magnetic beads. In the chamber, the magnetic beads are adsorbed. After adsorption, buffer or pure water i...

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Abstract

The invention relates to a microfluidic chip for nucleic acid extraction and PCR detection and its application. Microfluidic chip nucleic acid extraction reaction chamber, purification reaction chamber and PCR reaction chamber, the front end of the nucleic acid extraction reaction chamber and the purification reaction chamber are connected with two branch liquid inlet chambers, the back end is connected with a liquid extraction chamber, and the front end of the PCR reaction chamber is connected It is connected with a three-branch liquid inlet silo, and the rear end is connected with a liquid taking silo. The nucleic acid extraction reaction chamber and the purification reaction chamber can be made into one chip, the PCR reaction chamber is on another chip, and there can be one or more PCR reaction chambers. The microfluidic chip for nucleic acid extraction and PCR detection provided by the present invention effectively combines the nucleic acid extraction steps and the purification process with the PCR amplification reaction, realizes a fully sealed operation, avoids secondary pollution, and ensures the experimental results reliable, authentic and effective.

Description

technical field [0001] The invention relates to a microfluidic chip for nucleic acid extraction and PCR detection and its application, and belongs to the technical field of microfluidic chip analysis. Background technique [0002] Microfluidic chip technology (Microfluidics) is the basic operation unit of sample preparation, reaction, separation, detection, etc. in biological, chemical, and medical analysis processes. Analyze the whole process. Due to its huge potential in biology, chemistry, medicine and other fields, it has developed into a new research field that intersects with biology, chemistry, medicine, fluids, electronics, materials, machinery and other disciplines. Most of the existing microfluidic chips are composed of multi-layer or upper and lower layers of chips, and the chips are made of materials such as single crystal silicon, glass, quartz, and high molecular polymers. [0003] For the automatic sample change of conventional microfluidic chips, an array-t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00C12M1/40C12M1/38C12M1/00C12Q1/6806C12Q1/686
CPCB01L3/5027C12Q1/6806C12Q1/686B01L3/502761B01L2200/10B01L2300/0861B01L2400/043C12Q2523/308C12Q2565/629
Inventor 杨宇赖天然齐润姿周永运聂聪
Owner CHINESE ACAD OF INSPECTION & QUARANTINE
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