Rice seed seedling raising method based on combination of glomus intraradices and agrobacterium rhizogenes
A technology of intra-rhizobacterial fungus and Agrobacterium rhizogenes, which is applied in the directions of seed coating/seed dressing, rice cultivation, seed and rhizome treatment, etc., can solve the problems of poor application effect, etc. Pesticide and chemical fertilizer application rate, the effect of promoting growth and development
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specific Embodiment approach 1
[0007] Specific embodiment one: the present embodiment carries out the method for paddy seedling raising based on the combination of Glomus intraradices and A. 600 value of 0.6 to 0.8) diluted to 90 to 110 mL / L of Agrobacterium rhizogenes inoculum, soak the seeds in the inoculum for 8 to 16 hours, filter and then combine with fungal spores with a content greater than 25 / g The root capsule mold agent and the rice seedling-raising substrate are mixed evenly, and the seeds are sown, and the rice seedlings are raised.
[0008] The Agrobacterium rhizogenes in the present embodiment is a liquid inoculum, and the Agrobacterium rhizogenes inoculum is a pure bacterial liquid of Agrobacterium rhizogenes (bacterial liquid OD 600 The value is 0.6-0.8) diluted with sterile water to a liquid bacterial agent with a concentration of 90-110mL / L, and the bacterial agent is provided by the Key Laboratory of Ecological Restoration and Resource Utilization in Cold Regions of Heilongjiang Province....
specific Embodiment approach 2
[0011] Specific embodiment 2: The difference between this embodiment and specific embodiment 1 is that the weight ratio of rice seeds to Agrobacterium rhizogenes is 1:1.5-2.5. Other steps and parameters are the same as those in the first embodiment.
specific Embodiment approach 3
[0012] Specific embodiment 3: The difference between this embodiment and specific embodiment 1 is that the weight ratio of rice seeds to Agrobacterium rhizogenes is 1:2. Other steps and parameters are the same as those in the first embodiment.
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Abstract
Description
Claims
Application Information
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