A kind of cell preservation solution for liquid-based cell inspection and its preparation method

Abstract

The application relates to the technical field of early screening of cervical cancer, and specifically discloses a cell preservation solution for liquid-based cell inspection and a preparation method thereof. A cell preservation solution for liquid-based cell inspection includes a solute and a solvent, the solvent includes a phosphate buffered saline solution and a cell shape fixative, the solute includes a mucus-dissolving agent, an erythrocyte lysing agent, and a cell nucleus shape-fixing agent that can inhibit the activation of the CDK1 / CyclinB1 complex The preparation method is as follows: preparing phosphate buffered saline solution, adjusting the phosphate buffered saline solution to weak alkalinity, mixing it with cell shape fixative, and finally adding mucus decomposing agent, erythrocyte lysing agent and cell nucleus shape fixing agent for mixing. The cell preservation solution disclosed in the present application can be used for the preservation and treatment of sample cells in LBC technology, and has the advantages of maintaining the integrity of the nucleus morphology and improving the staining effect of the substances in the nucleus.

Description

technical field [0001] This application relates to the technical field of early screening of cervical cancer, more specifically, it relates to a cell preservation solution for liquid-based cell examination and a preparation method thereof. Background technique [0002] Cervical cancer is one of the more common and frequent malignant tumors in clinical gynecology, and it is also an important disease that causes increased mortality among women. Its incidence rate is second only to breast cancer. About 30,000 people die of cervical cancer every year in my country, and cervical cancer The number of patients who die of illness is increasing year by year. Fortunately, cervical cancer is currently the only gynecological malignancy with a clear etiology, which is related to the persistent infection of high-risk human papillomavirus (HPV). Therefore, the occurrence and development of cervical cancer require a gradual evolution process. The duration of cervical cancer can range from s...

AI Technical Summary

Problems solved by technology

[0004] However, there are still many problems in the current LBC technology. A common problem is that the staining effect of the sample cells on the prepared smear is poor after staining, which increases the difficulty of diagnosis and the technical requirements for diagnostic personnel.

refer to figure 1 and figure 2 At present, the staining effect of the nuclei in the sample cells on the smears obtained by LBC technology is poor, resulting in relatively uniform color of the stained nuclei, and the chromatin in the nuclei cannot be displayed clearly, which makes doctors Difficult to distinguish diseased cells

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