Method for extracting and determining sulfonamide antibiotics in solid environment sample by using water solvent

Abstract

The invention belongs to the field of antibiotic detection, and relates to a method for extracting and determining sulfonamide antibiotics in a solid environment sample by a water solvent. The method comprises a sample extraction step. The sample extraction step comprises the following steps of: taking pure water as an extracting agent, and extracting the sulfonamide antibiotics under the conditions that the temperature is greater than or equal to 80 DEG C and the pressure is greater than or equal to 101.325 kPa. Extraction is carried out at high temperature and high pressure, phase change does not need to be carried out on the extracting solution after extraction is completed, thus, time is saved, and the situation that the measurement accuracy is affected by inevitable loss of a target measured object in the phase change process is avoided. The determination method provided by the invention is a green extraction method, no organic solvent is involved in the extraction process, no pollution is caused to the environment, the overall operation time is also greatly shortened, and rapid separation and detection of at least 10 sulfonamide antibiotics can be completed within 10 minutes.

Description

technical field [0001] The invention belongs to the field of antibiotic detection, and more specifically relates to a method for extracting and measuring sulfonamide antibiotics in solid-state environmental samples by water solvent. Background technique [0002] Antibiotics refer to chemical substances produced by biological actions that have anti-pathogen or other types of secondary biological metabolites and other synthetic and semi-synthetic substances that can act on living cells. In 2016, my country's annual consumption reached 180,000 tons, and its role involves agriculture, animal husbandry, medical care, and breeding industries. With the abuse of antibiotics, its environmental problems have become increasingly serious, and the harm caused by antibiotic residues in environmental media has become a hot spot in the 21st century. The establishment of its antibiotic residue detection method has important practical significance for the migration and transformation of anti...

AI Technical Summary

Problems solved by technology

[0004] For the defect of prior art, the object of the present invention is to provide a kind of method simple and green and the assay method of sulfa antibiotics in the solid-state environmental sample that measurement result is accurate, and it utilizes the characteristic that sulfa antibiotics itself has certain polarity, adopts pure water As an extractant, it is extracted under high temperature and high pressure, aiming to solve the technical problems that the extractant contains organic solvents and other complex components in the existing sulfonamide antibiotic determination method, which leads to cumbersome separation and enrichment steps and affects the detection accuracy.

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