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A kind of method for live inoculation of pecan fruit with thin shell

A thin-shell hickory and live inoculation technology, which is applied in the field of bioengineering, can solve the problems of easy breakage of the sealing film, long operation time, and high difficulty of operation, and achieve the effects of high separation rate, easy operation, and low cost

Active Publication Date: 2022-03-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The conventional live inoculation method of pecan fruit with thin shells is to connect the fungus cake to the fruit, seal the fungus cake and wind it on the fruit with a sealing film, but it is difficult to operate, the operation time is long, and the sealing film is easy to dry out. Fracture and other issues

Method used

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  • A kind of method for live inoculation of pecan fruit with thin shell
  • A kind of method for live inoculation of pecan fruit with thin shell
  • A kind of method for live inoculation of pecan fruit with thin shell

Examples

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Effect test

Embodiment 1

[0032] Different types of shell pecan varieties including: Taitian, Gaografting, Mahan, Pawnee, Yalin, Shaoxing, Jinhua, Stewart, Cardo, Wichita. The thin-shell pecan variety adopted in the present embodiment is Jinhua.

[0033] A method for live inoculation of pecan fruit with thin shells, comprising the following steps:

[0034] 1. Selection of strains:

[0035] Transplant the Trichosporum pseudodiscoides species preserved after isolation and purification in the field to a new PDA medium for activation, and culture at 25° C. to 28° C. for 3-5 days until the hyphae cover the entire medium.

[0036] The preparation method of PDA medium is as follows: take 200 g of washed and peeled potatoes, cut into pieces, add water and boil for 20 minutes, filter the potatoes with gauze, then add 20 g of agar, heat until the agar is completely melted, then add 20 g of glucose, add water Set the volume to 1000ml, then divide it into Erlenmeyer flasks, put it into a high-pressure steam ster...

Embodiment 2

[0057] It is basically the same as Example 1, except that Trichosporum is replaced by Bacillus anthracis.

Embodiment 3

[0059] It is basically the same as in Example 1, except that Phomopsis is replaced by Phomopsis.

[0060] Such as Figure 1 to Figure 3 Shown, respectively, use this method to inoculate Phomopsis, anthracnose, and Trichosanthes 5-7 days after the inoculation of Phomopsis, anthracnose, and Trichosporum, respectively. Walnut fruit, followed by anthracnose and Phomopsis. Small black spots appeared in the early stages of the disease on fruits inoculated with Phomopsis and Phomopsis, and gradually expanded in the middle stage, and the black spots showed irregular circles until they expanded into the whole fruit, and the inside of the fruit turned black and rotted, and the fruit fell off. In the early stage of the disease, the surface of the fruit inoculated with anthracnose has soft raised brown spots, and the mid-term lesion gradually expands and turns brown until the whole fruit. The middle of the lesion turns reddish brown, and the damaged part inside the fruit turns brown.

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Abstract

The invention discloses a method for live inoculation of pecan fruit with thin shells, and belongs to the technical field of bioengineering. The method comprises the following steps: 1) selecting healthy fruits on hickory trees with thin shells, disinfecting them, and stabbing the fruits for use; One side of the mycelium is close to the puncture wound of the fruit; use the fungus cake covering device to cover the fungus cake and press firmly to make it stick to the fruit; 3) Investigate the incidence of the inoculation point. Wherein, the bacterial cake covering device includes covering the tube body, covering the tube cap and sterile absorbent cotton, the bottom of the covering tube body is closed, and is provided with compacted sterile absorbent cotton in a water-saturated state, controlled by covering the tube cap Covers the open and closed state of the tube body. Using the bacteria cake covering device of the present invention can keep the bacteria cake moist, and can prevent other pathogenic bacteria from outside from interfering with the results, and the separation rate of pathogenic bacteria strains from diseased tissues is high.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a method for live inoculation of pecan fruit with thin shells. Background technique [0002] Thin shell hickory (Carya illinoinensis (Wangenh.) K. Koch), also known as American hickory, long hickory, Juglandaceae (Juglandaceae) Hickory (Carya) deciduous trees. It is an excellent greening tree species, high-quality military timber, and its fruit is an ideal health product. The tree shape is tall and straight, the root system is developed, and it is resistant to water and humidity. It combines dried fruits, oil plants, greening and materials, and has good economic, ecological and social benefits. It has been introduced to my country for more than 100 years, and it is an excellent economic tree species integrating economic, social and ecological benefits. [0003] In recent years, with the improvement of people's quality of life, the demand for nuts has graduall...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12Q1/18A01H4/00
CPCA01H4/001A01H4/008
Inventor 吴天昊巨云为
Owner NANJING FORESTRY UNIV
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