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S-adenosyl homocysteine artificial complete antigen as well as preparation method and application thereof

A cysteine ​​and adenosine homotype technology, applied in the preparation of S-adenosine homocysteine ​​artificial complete antigen, the application of S-adenosyl homocysteine ​​artificial complete antigen, S-adenosine homocysteine The field of cysteine ​​artificial complete antigen can solve the problems of weak immunogenicity and poor specificity of S-adenosyl homocysteine ​​artificial complete antigen

Pending Publication Date: 2021-06-22
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, it is necessary to provide a preparation method of S-adenosylhomocysteine ​​artificial complete antigen to solve the above-mentioned problems caused by the preparation method of existing S-adenosylhomocysteine ​​artificial complete antigen The S-adenosylhomocysteine ​​artificial complete antigen has the disadvantages of weak immunogenicity and poor specificity

Method used

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  • S-adenosyl homocysteine artificial complete antigen as well as preparation method and application thereof
  • S-adenosyl homocysteine artificial complete antigen as well as preparation method and application thereof
  • S-adenosyl homocysteine artificial complete antigen as well as preparation method and application thereof

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preparation example Construction

[0052] see figure 1 , the embodiment of the present invention provides a preparation method of S-adenosylhomocysteine ​​artificial complete antigen, comprising the following steps:

[0053] Step S1: providing S-adenosylhomocysteine ​​(SAH), amino-protecting agent (Fmoc), resin carrier, and carrier protein;

[0054] Step S2: mixing the S-adenosylhomocysteine ​​and the amino-protecting agent, the S-adenosylhomocysteine ​​and the amino-protecting agent undergo a nucleophilic reaction to obtain the first intermediate (abbreviated as Fmoc-SAH );

[0055] Step S3: mixing the first intermediate and the resin carrier, the first intermediate and the resin carrier undergo an esterification reaction to obtain a second intermediate;

[0056] Step S4: removing the amino protecting group on the second intermediate to obtain a third intermediate;

[0057] Step S5: mixing the first intermediate and the third intermediate, the first intermediate and the third intermediate undergo a polymeri...

Embodiment 1

[0118] Dissolve 1mg of sodium carbonate in 10mL of deionized water, add 0.26mmol of S-adenosylhomocysteine ​​after the sodium carbonate is completely dissolved, slowly add 2.5mL of tetrahydrofuran and 2.5mL of dioxane in an ice-bath environment, dropwise After completion, stir magnetically at 0°C for 15 minutes. After S-adenosylhomocysteine ​​is completely dissolved, add 2.5 times the molar equivalent of 9-fluorenylmethoxycarbonyl chloride, continue the reaction at 0°C for 30 minutes, and then heat up React at 25°C for 12 hours in a dark environment. After the reaction, add 10 mL of diethyl ether to extract three times, separate the water phase, adjust the pH to 3, let it stand for 3 minutes, filter the precipitated crystals, and recover the crystals by freeze-drying with a lyophilizer to obtain the first intermediate;

[0119] Add 20mg of Wang resin into 10mL of dichloromethane, soak and swell for 30 minutes, filter and dry to recover for use;

[0120] Dissolve 54.5 μmol of ...

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Abstract

The invention provides a preparation method of the S-adenosyl homocysteine artificial complete antigen. The method comprises the following steps: mixing S-adenosylhomocysteine and an amino protective agent, and carrying out condensation reaction to obtain a first intermediate; mixing the first intermediate and a resin carrier, and carrying out esterification reaction to obtain a second intermediate; removing an amino protecting group on the second intermediate to obtain a third intermediate; mixing the first intermediate and the third intermediate, and carrying out polymerization reaction to obtain a fourth intermediate; removing an amino protecting group on the fourth intermediate to obtain a fifth intermediate; stripping the resin carrier on the fifth intermediate to obtain a sixth intermediate; and mixing the sixth intermediate with a carrier protein, and coupling the sixth intermediate with the carrier protein to obtain the S-adenosine homocysteine artificial complete antigen. The S-adenosine homocysteine artificial complete antigen prepared by the preparation method of the S-adenosine homocysteine artificial complete antigen disclosed by the invention has the advantages of strong immunogenicity and good specificity.

Description

technical field [0001] The present invention relates to the technical field of artificial antigens, in particular to a method for preparing an S-adenosylhomocysteine ​​artificial complete antigen. S-adenosyl homocysteine ​​artificial complete antigen and application of the S-adenosyl homocysteine ​​artificial complete antigen. Background technique [0002] S-adenosylhomocysteine ​​belongs to hapten and has no immunogenicity by itself. It needs to be coupled with a suitable carrier protein to prepare the artificial complete antigen of S-adenosylhomocysteine. However, the S-adenosylhomocysteine ​​produced by the existing S-adenosylhomocysteine ​​artificial complete antigen preparation method has the disadvantage of antigen instability, which in turn leads to the production of S-adenosylhomocysteine Homocysteine ​​artificial complete antigen has the disadvantages of weak immunogenicity and poor specificity. Contents of the invention [0003] In view of this, it is necessary...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/765C07K14/77C07K14/795C07K1/04C07K1/06C07K1/10C07K1/107C07K16/44
CPCC07K14/765C07K14/77C07K14/795C07K16/44Y02P20/55
Inventor 马岚刘丹吴峰岑瑜
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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