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A strain of Bifidobacterium adolescentis that promotes the proliferation of animal bifidobacteria and its application

A technology of animal bifidobacteria and bifidobacteria, applied in the field of microorganisms, can solve the problems of long cycle, complicated process, unsuitable for the proliferation of animal bifidobacteria, etc., and achieve the effect of improving utilization ability and promoting proliferation

Active Publication Date: 2022-07-01
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, Watson et al. have found that some animal bifidobacteria have weak ability to utilize inulin, and only supplementing inulin cannot promote their proliferation (disclosed in D Watson, Motherway M O, Schoterman M, et al.Selectivecarbohydrate utilization by lactobacilli and bifidobacteria[ J]. Journal of Applied Microbiology, 2013, 114(4). In the paper)
[0008] It can be seen that the method for promoting the proliferation of animal bifidobacteria by supplementing inulin is not effective, and the publication number is CN104928215A In the Chinese invention application document, after the bacterial strain is enriched and cultivated in a carbon dioxide incubator, it is continued in the fermentation medium. Cultivation, the cycle is as long as 8 days, just can increase the biomass of bifidobacterium animal, but, this method cycle is long, process is complicated, also is not suitable for the proliferation of bifidobacterium animal in vivo, therefore, urgently needs to find a kind of can further improve Method for in vivo and in vitro propagation of animal bifidobacteria

Method used

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  • A strain of Bifidobacterium adolescentis that promotes the proliferation of animal bifidobacteria and its application
  • A strain of Bifidobacterium adolescentis that promotes the proliferation of animal bifidobacteria and its application
  • A strain of Bifidobacterium adolescentis that promotes the proliferation of animal bifidobacteria and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0075] Example 1: Isolation, screening and identification of Bifidobacterium adolescentis CCFM1169

[0076] Specific steps are as follows:

[0077] 1. Isolation and screening of Bifidobacterium adolescentis CCFM1169

[0078] (1) 1 mL of fecal samples from middle-aged and elderly people collected from different areas in Jinan, Shandong Province (the fecal samples were stored in 30% glycerol) were drawn, and the fecal samples were spread on the modified MRS solid medium after gradient dilution. The anaerobic workstation was cultured for 48 hours, and the temperature of the anaerobic workstation was 37 °C;

[0079] (2) Pick the strains with differences in colony morphology, carry out streak separation and purification on the modified MRS solid medium, and record the morphological characteristics of the bacteria in detail (the results are shown in Table 1);

[0080] (3) picking the single bacteria isolated from the culture medium in step (2), transferring it to the modified MRS ...

Embodiment 2

[0096] Example 2: Growth of different Bifidobacterium strains in different carbon source media

[0097] In this example, the inventors used Bifidobacterium adolescentis CCFM1169, Bifidobacterium adolescentis CCFM1066, Bifidobacterium animalis CCFM1148, Bifidobacterium animalis CCFM624 and Bifidobacterium animalis CCFM625 of the present invention as The research subjects, with inulin, oligofructose, fructose and glucose as the only carbon sources, were cultured in micropores in carbon source-limited medium to evaluate their utilization of different carbon sources.

[0098] Specific steps are as follows:

[0099] (1) Preparation of culture medium

[0100] Glucose liquid medium: the modified MRS liquid medium is used;

[0101] Sugar-free liquid medium: the modified MRS liquid medium without glucose is used;

[0102] Fructose liquid medium: replace the glucose in the modified MRS liquid medium with fructose;

[0103] Fructooligosaccharide liquid medium: Replace the glucose in ...

Embodiment 3

[0116] Example 3: Growth of 5 Bifidobacterium strains in inulin medium containing 1% and 0.5% respectively

[0117] In this example, the inventors used Bifidobacterium adolescentis CCFM1169, Bifidobacterium adolescentis CCFM1066, Bifidobacterium animalis CCFM1148, Bifidobacterium animalis CCFM624 and Bifidobacterium animalis CCFM625 of the present invention as The research subjects, with 1% and 0.5% inulin as the sole carbon source, were cultured in micropores in a carbon source-limited medium to evaluate the effects of different contents of inulin on their growth.

[0118] Specific steps are as follows:

[0119] (1) Preparation of seed solution

[0120] The preparation method is the same as the step (2) of Example 2, except that the adjusted Bifidobacterium strains are Bifidobacterium adolescentis CCFM1066 and Bifidobacterium adolescentis CCFM1169, as well as Bifidobacterium animalis CCFM1148, Bifidobacterium animalis CCFM624 and Bifidobacterium animalis Bifidobacterium ado...

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Abstract

The invention discloses a Bifidobacterium adolescentis strain promoting the proliferation of animal Bifidobacterium and an application thereof, belonging to the technical field of microorganisms and medical technology. The present invention provides a strain of Bifidobacterium adolescentis CCFM1169. The Bifidobacterium adolescentis CCFM1169 is preserved in the Guangdong Provincial Microbial Culture Collection Center, and the preservation number is GDMCC No: 61547, and the preservation date is March 09, 2021. The bifidobacteria adolescentis of the invention can improve the utilization rate and growth rate of bifidobacteria animal to inulin, and the colony number of bifidobacteria animal in the fermentation liquid is increased. Animal experiments show that the Bifidobacterium adolescentis can promote the proliferation of Bifidobacterium animalis in the intestinal tract of C57BL / 6J mice, which has great application and commercial value.

Description

technical field [0001] The invention relates to a Bifidobacterium adolescentis strain that promotes the proliferation of animal Bifidobacterium and its application, belonging to the technical field of microorganisms and the technical field of medicine. Background technique [0002] Bifidobacterium animalis is a common intestinal microorganism. It plays an important role in maintaining intestinal balance, inhibiting the growth and reproduction of harmful microorganisms in the intestinal tract, resisting pathogen infection, and enhancing body immunity. Studies have shown that when there are high concentrations of animal bifidobacteria in the intestinal tract, external invading pathogens such as Escherichia coli, Shigella dysenteriae, Salmonella typhi, Proteus, Staphylococcus aureus, Candida albicans or internal The source of saprophytic bacteria, opportunistic pathogenic bacteria will be inhibited. In addition, bifidobacteria can produce short-chain fatty acids such as acetic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L33/135A23C9/123A23C9/152A23C9/16A23C17/02A61K35/745A61P1/00C12R1/01
CPCC12N1/20A23L33/135A23C9/1234A23C9/152A23C9/16A23C17/02A61K35/745A61P1/00A23V2002/00A23V2400/513A23V2200/3204
Inventor 翟齐啸骆燕红陈卫肖越于雷雷田丰伟陆文伟崔树茂王刚赵建新张灏
Owner JIANGNAN UNIV
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