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Hollow tube bundle type fixed bed bioreactor for adherent culture of mammalian cells

A technology of bioreactor and adherent culture, which is applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, method of supporting/immobilizing microorganisms, etc., which can solve the problem of uneven cell distribution, shearing Eliminate problems such as cell damage and difficult cell harvesting, and achieve the effects of improving culture efficiency, promoting oxygen mass transfer, and promoting uniform attachment

Pending Publication Date: 2021-06-18
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In view of the above problems, the present invention provides a hollow tube-bundle fixed-bed bioreactor for adherent culture of mammalian cells, which can solve the problem of uneven cell distribution and easy occurrence of center of carrier stacking during the use of existing fixed-bed bioreactors. "Dead zone", the technical problem that the shear force seriously damages the cells and the cells are difficult to harvest

Method used

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  • Hollow tube bundle type fixed bed bioreactor for adherent culture of mammalian cells
  • Hollow tube bundle type fixed bed bioreactor for adherent culture of mammalian cells
  • Hollow tube bundle type fixed bed bioreactor for adherent culture of mammalian cells

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Effect test

Embodiment 1

[0024] A hollow tube bundle fixed bed bioreactor for adherent culture of mammalian cells, such as figure 1 , Figure 2a , Figure 2b As shown, it includes a tank body 1, a carrier 5, and a cultivation and driving unit 4. A flange cover 2 is installed on the top of the tank body 1, a base 3 is installed on the bottom, and a carrier 5 is installed inside. And evenly distributed flow passages, gaps are respectively provided between the upper end of the carrier 5 and the flange cover 2, and between the carrier 5 and the side wall of the tank body 1, and the cultivation and driving unit 4 is a low-shear jet pump (low-shear jet pump) The injection direction of the culture medium of the force jet pump is from top to bottom, that is, towards the axis of the tank body and the flow channel. The access of the actual structure of the pump does not affect the actual implementation of the invention), the low-shear jet pump is fixedly installed on the base 3, and during use, as Figure 4 ...

Embodiment 2

[0030] Such as Figure 3a , Figure 3b As shown, the difference between Embodiment 2 and Embodiment 1 is that a plurality of tube bodies 52 that are parallel to each other and fit together are surrounded and fixed by binding members 51 to form a circular tube bundle 53, and multiple tube bundles 53 are parallel to each other and fit together. Afterwards, it is surrounded and fixed by the binding member 51 to form a circular carrier.

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Abstract

The invention provides a hollow tube bundle type fixed bed bioreactor for adherent culture of mammalian cells, and can effectively solve the technical problems that cells are unevenly distributed on a carrier, the center of a carrier stack is prone to produce a cell-free "dead zone", shear force of a fluid medium in the reactor is large, and the cells are difficult to harvest. The hollow tube bundle type fixed bed cell bioreactor comprises a tank body, a carrier, a culture medium driving unit and a gas supply unit; a flange cover is mounted at the top of the tank body, and the carrier is arranged in the tank body. The hollow tube bundle type fixed bed cell bioreactor is characterized in that only flow channels vertical and evenly distributed are arranged in the carrier; gaps are arranged between the upper end of the carrier and the flange cover and between the carrier and the side wall of the tank body; and culture mediums in the tank body flow in from the opening at lower ends of the flow channels under the action of the culture medium driving unit, and then return to the bottom of the tank body after sequentially passing through the flow channels, the gap between the carrier and the flange cover and the gap between the carrier and the side wall of the tank body.

Description

technical field [0001] The invention relates to the technical field of cell biological culture devices, in particular to a hollow tube-bundle fixed-bed bioreactor for adherent culture of mammalian cells, which is used in the research and development and production process of biomedical products. Background technique [0002] The large-scale culture of adherent cells used in the production of virus vaccines, virus vectors, therapeutic antibodies and other protein drugs mainly uses stirred bioreactors (microcarrier suspension culture) and fixed bed bioreactors (mostly medical high sheet-like carrier made of molecular or fibrous material). Compared with microcarrier suspension culture, the fixed bed bioreactor fixes the carrier in a certain area in the reactor, and the cells attach to the surface of the carrier and grow inside the carrier fiber, thus avoiding the shear force caused by stirring and carrier collision; Amplification and migration between carriers can be realized,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M3/00
CPCC12M25/10C12M25/18
Inventor 白仲虎聂简琪郭永信李康孙杨
Owner JIANGNAN UNIV
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