A slow-release algae-inhibiting microsphere and its preparation method and application
A microsphere and slow-release technology, applied in the field of slow-release anti-algae microspheres and its preparation, can solve the problem of lack of long-term and high-efficiency inhibition effect of microcystin MCs, long-term and stable anti-algae growth effect, and complex production process and other problems, to achieve the effect of improving algae inhibition effect, embedding efficiency, and simple preparation process
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Embodiment 1
[0035] The present embodiment provides a slow-release algae-inhibiting microsphere, the raw materials of which include:
[0036]
[0037] Note: the above denominator v is the volume of the mixture of luteolin emulsification system and sodium alginate described in the preparation method of slow-release algae-inhibiting microspheres.
[0038] Chitosan 0.25%(w / v)
[0039] Calcium chloride 4% (w / v)
[0040] Note: The denominator v above (chitosan, calcium chloride) is the volume of the aqueous solution of chitosan and calcium chloride described in the preparation method of slow-release algae-inhibiting microspheres.
[0041] Glutaraldehyde 0.5% (v / v, denominator v is the total volume after the mixed solution of luteolin and sodium alginate is dropped into the aqueous solution of chitosan and calcium chloride described in the preparation method of slow-release algae-inhibiting microspheres ).
[0042] The preparation method of the above-mentioned slow-release algae-inhibiting...
Embodiment 2
[0049] Test of luteolin release characteristics of slow-release algae-inhibiting microspheres provided in Example 1:
[0050] Put 0.02 g / mL slow-release algae-inhibiting microspheres (containing 0.41 mg / mL luteolin equivalent) into the medium, and culture at 25±1 °C and 2000 lux of light (12 h light / 12 h dark).
[0051] By replacing the same amount of fresh medium every 1.5 days, the cumulative release of luteolin could be measured. The results showed that the release of luteolin increased to 171.79 μg / mL on the first day, and then decreased and stabilized at a lower level. Until the 49th day, there was a slight increase on the 10th to 13th day. The cumulative release amount from 22 to 49 days was 300.27-383.58 μg / mL, accounting for 73.32%-81.36% of the total content of luteolin in slow-release algae-inhibiting microspheres.
[0052] The actual concentration of luteolin released can be measured by sampling regularly without changing the medium. The results show that the avera...
Embodiment 3
[0054] The high-efficiency and lasting inhibitory effect of the slow-release algae-inhibiting microspheres provided in Example 1 on the growth of Microcystis was tested:
[0055] Tested algal species: Microcystis aeruginosa FACHB-915.
[0056] Methods: Microcystis in exponential growth phase was inoculated into BG11 medium, and the initial density of algal cells was 4×10 5 cells / mL, which is in line with the algal density during the bloom of Microcystis. According to the dose of luteolin in the slow-release algae-inhibiting microspheres and its release characteristics, the slow-release algae-inhibiting microspheres were added to the Microcystis culture solution to make The concentration reaches 0.004g / mL, and at the same time, it is set to contain 0.082mg / mL pure luteolin (equivalent to luteolin contained in slow-release algae-inhibiting microspheres) and 0.004g / mL blank microspheres (without luteolin). The microcystis culture solution and other treatment groups, and the Micr...
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