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A slow-release algae-inhibiting microsphere and its preparation method and application

A microsphere and slow-release technology, applied in the field of slow-release anti-algae microspheres and its preparation, can solve the problem of lack of long-term and high-efficiency inhibition effect of microcystin MCs, long-term and stable anti-algae growth effect, and complex production process and other problems, to achieve the effect of improving algae inhibition effect, embedding efficiency, and simple preparation process

Active Publication Date: 2022-05-24
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The algae inhibitor slow-release technology uses physical and chemical means to store effective algae-inhibiting ingredients in continuous-release microspheres (continuous-release microspheres, CRM), and then release them slowly and continuously. The preparation of existing CRM and its anti-algae The effect has the following disadvantages: the production process is complicated and the production cost is high; the effect of inhibiting algae growth cannot be achieved for a long time and stable, and the effect needs to be further strengthened; while inhibiting the growth of algae, it lacks a long-term and high-efficiency inhibitory effect on the release of microcystin MCs; There is no report on the effect of CRM on inhibiting algae and inhibiting MCs under different environmental conditions

Method used

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  • A slow-release algae-inhibiting microsphere and its preparation method and application
  • A slow-release algae-inhibiting microsphere and its preparation method and application
  • A slow-release algae-inhibiting microsphere and its preparation method and application

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Embodiment 1

[0035] The present embodiment provides a slow-release algae-inhibiting microsphere, the raw materials of which include:

[0036]

[0037] Note: the above denominator v is the volume of the mixture of luteolin emulsification system and sodium alginate described in the preparation method of slow-release algae-inhibiting microspheres.

[0038] Chitosan 0.25%(w / v)

[0039] Calcium chloride 4% (w / v)

[0040] Note: The denominator v above (chitosan, calcium chloride) is the volume of the aqueous solution of chitosan and calcium chloride described in the preparation method of slow-release algae-inhibiting microspheres.

[0041] Glutaraldehyde 0.5% (v / v, denominator v is the total volume after the mixed solution of luteolin and sodium alginate is dropped into the aqueous solution of chitosan and calcium chloride described in the preparation method of slow-release algae-inhibiting microspheres ).

[0042] The preparation method of the above-mentioned slow-release algae-inhibiting...

Embodiment 2

[0049] Test of luteolin release characteristics of slow-release algae-inhibiting microspheres provided in Example 1:

[0050] Put 0.02 g / mL slow-release algae-inhibiting microspheres (containing 0.41 mg / mL luteolin equivalent) into the medium, and culture at 25±1 °C and 2000 lux of light (12 h light / 12 h dark).

[0051] By replacing the same amount of fresh medium every 1.5 days, the cumulative release of luteolin could be measured. The results showed that the release of luteolin increased to 171.79 μg / mL on the first day, and then decreased and stabilized at a lower level. Until the 49th day, there was a slight increase on the 10th to 13th day. The cumulative release amount from 22 to 49 days was 300.27-383.58 μg / mL, accounting for 73.32%-81.36% of the total content of luteolin in slow-release algae-inhibiting microspheres.

[0052] The actual concentration of luteolin released can be measured by sampling regularly without changing the medium. The results show that the avera...

Embodiment 3

[0054] The high-efficiency and lasting inhibitory effect of the slow-release algae-inhibiting microspheres provided in Example 1 on the growth of Microcystis was tested:

[0055] Tested algal species: Microcystis aeruginosa FACHB-915.

[0056] Methods: Microcystis in exponential growth phase was inoculated into BG11 medium, and the initial density of algal cells was 4×10 5 cells / mL, which is in line with the algal density during the bloom of Microcystis. According to the dose of luteolin in the slow-release algae-inhibiting microspheres and its release characteristics, the slow-release algae-inhibiting microspheres were added to the Microcystis culture solution to make The concentration reaches 0.004g / mL, and at the same time, it is set to contain 0.082mg / mL pure luteolin (equivalent to luteolin contained in slow-release algae-inhibiting microspheres) and 0.004g / mL blank microspheres (without luteolin). The microcystis culture solution and other treatment groups, and the Micr...

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Abstract

The invention provides a slow-release algae-inhibiting microsphere, a preparation method and application thereof. The slow-release algae-inhibiting microspheres are prepared from the following raw materials: luteolin 1%-1.5% (w / v), olive oil 18%-20% (w / v), emulsifier 10%-12% (w / v), co-emulsifier 10%~12% (w / v), sodium alginate 1%~2.5% (w / v), chitosan 0.2%~0.3% (w / v), chloride Calcium 4%-8% (w / v), cross-linking agent 0.5%-1% (v / v). The slow-release algae-inhibiting microspheres provided by the present invention can slow down and control the release rate of luteolin, and the preparation process is simple, the cost is low, and there is no pollution to the environment. Through the sustained and slow release of luteolin, it can significantly It effectively inhibits the growth of Microcystis and at the same time effectively inhibits the release of microcystins (MCs), without the need to repeatedly add algicides, and has a long-term and stable effect on effectively inhibiting the outbreak of cyanobacteria blooms.

Description

technical field [0001] The invention relates to the technical field of water environment protection, in particular to a slow-release algae-inhibiting microsphere and a preparation method and application thereof. Background technique [0002] In recent years, cyanobacterial blooms have occurred frequently in many large lakes and reservoirs. Microcystis is the dominant species for blooms, which can damage aquatic ecosystems by blocking sunlight and consuming oxygen. Microcystis usually produces and releases algal toxins to further pollute water bodies, poisoning aquatic organisms and humans, among which microcystins (MCs) are the most representative. The World Health Organization recommends that the content of MCs in drinking water be set at 1 μg / L, so controlling the growth of harmful algae and reducing the release of MCs has become a widespread concern. [0003] The management of harmful algae can be divided into physical, chemical and biological methods. However, water tr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N25/28A01N43/16A01P13/00B01J13/14
CPCA01N25/28A01N43/16B01J13/14
Inventor 李洁明李碧滢安广琪
Owner CHINA AGRI UNIV
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