Extraction method and application of bonded aroma substance in mango peel
A technology of aroma substances and extraction methods, which is applied in the field of extraction of bonded aroma substances in mango peel, can solve the problems of lightening aroma, harmfulness to human body, loss of mango juice aroma, etc. The effect of increasing content
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Embodiment 1
[0035] This embodiment provides a method for extracting bonded aroma substances in mango peel, the method comprising the following steps:
[0036] (1) Prepare mango peel juice: take mango peel and carry out crushing process to obtain mango peel pulp, mango peel pulp is centrifuged to get supernatant, and mango peel juice is obtained for subsequent use;
[0037] (2) Elution: Adsorb the mango peel juice on Amberlite XAD-2 resin, elute the bonded aroma substances in the mango juice with 2.5BV of anhydrous methanol under pressurized state, and elute it under 0.1Mpa Anhydrous methanol was removed by rotary evaporation, and the obtained eluate was concentrated; the above concentration was concentrated under reduced pressure at a temperature of 45°C;
[0038] (3) Drying: Freeze-dry the concentrated eluate obtained in step (2) in a freeze dryer to obtain the bonded aroma substances in the mango peel; wherein, the freeze-drying conditions are: temperature is -38 ° C, pressure is 16 Pa ...
Embodiment 2
[0040] This embodiment provides a method for extracting bonded aroma substances in mango peel, the method comprising the following steps:
[0041] (1) Prepare mango peel juice: take mango peel and carry out crushing process to obtain mango peel pulp, mango peel pulp is centrifuged to get supernatant, and mango peel juice is obtained for subsequent use;
[0042](2) Elution: Adsorb the mango peel juice on the Amberlite XAD-2 resin, elute the bonded aroma substances in the mango juice with 2.5BV of anhydrous methanol under pressure, and elute it under 0.2Mpa Anhydrous methanol was removed by rotary evaporation, and the obtained eluate was concentrated; the above concentration was concentrated under reduced pressure at a temperature of 55°C;
[0043] (3) Drying: Freeze-dry the concentrated eluent obtained in step (2) in a freeze dryer to obtain the bonded aroma substances in the mango peel; wherein, the freeze-drying conditions are: temperature is -43°C, pressure is 30pa .
Embodiment 3
[0045] This embodiment provides a method for preparing mango juice. The flavoring agent used in the method is the mango peel bonded aroma substance described in Example 1, which specifically includes the following steps:
[0046] 1) Mango raw juice preparation: peel and remove the core of the mango and squeeze the pulp into juice, then place the obtained juice at a temperature of 25°C for 5 hours to obtain the mango raw juice for later use;
[0047] 2) The first enzymolysis: Add 0.5 parts by weight of bonded aroma substances to 55 parts by weight of mango juice to obtain aroma-enhancing mango juice, then add pectinase to the aroma-enhancing mango juice for enzymolysis to obtain One enzymatic hydrolysis of fruit juice; as mentioned above, the amount of pectinase added is 0.01% of the mass of mango juice for flavoring; pectinase enzymolysis is carried out at 28°C for 40 minutes;
[0048] 4) Second enzymatic hydrolysis: Add β-glucosidase to the first enzymatic hydrolysis fruit ju...
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