A method for efficient propagation of bamboo seeds as explants

Abstract

The invention belongs to a method for efficient breeding of explants using square bamboo seeds, comprising the following steps: selecting square bamboo seeds just harvested in early May as explant materials, after pretreatment, disinfection, inoculation, cluster bud induction, Cultivate and root to generate tissue culture shoots. The invention fills up the blank of obtaining cluster seedlings through the rapid propagation of seeds as explants of square bamboo seeds, uses the seeds of square bamboo as explant materials, and combines the combination of different growth regulators to design a medium suitable for inducing cluster buds and rooting of square bamboo , It only takes about 20 weeks to complete the rooting of square bamboo seeds, and a large number of cluster buds can be obtained in a short period of time, which effectively solves the difficulty in the propagation of square bamboo, and overcomes the traditional shortcomings of low transplanting efficiency and high transportation costs of the mother plant.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency breeding method using bamboo seeds as explants. Background technique [0002] The genus Chimonobambusa was established by Japanese scholar Tomitaro Makino in 1914. At that time, there was only one species of Chimonabambusa. Han bamboo is native to my country and Japan, and was introduced to England as early as the 19th century. Bamboo species of the genus Phyllostachys have gradually adapted to cold, humidity-loving and shade-tolerant environments during the long-term evolution process. Therefore, they are mainly distributed in middle and high altitude mountainous areas. Fang bamboo is naturally distributed in the provinces south of the Qinling Mountains and southern Tibet, but the concentrated distribution areas are in Guizhou, Sichuan, Chongqing and Yunnan provinces. It extends southward to Vietnam and Burma, and eastward to Japan. ...

AI Technical Summary

Problems solved by technology

At present, most of the ways of bud propagation of bamboo adopt the method of stalk segment, but there is no report on the method of efficient and rapid propagation of bamboo seeds as explants

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