Efficient breeding method capable of taking Chimonobambusa quadrangularis (Fenzi) Makino seed as explant

A technology of bamboo seeds and explants, applied in the field of plant tissue culture

Active Publication Date: 2021-06-08
NANJING FORESTRY UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, most of the ways of bud propagation of bamboo adopt the method of stalk segment, ...

Method used

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  • Efficient breeding method capable of taking Chimonobambusa quadrangularis (Fenzi) Makino seed as explant
  • Efficient breeding method capable of taking Chimonobambusa quadrangularis (Fenzi) Makino seed as explant
  • Efficient breeding method capable of taking Chimonobambusa quadrangularis (Fenzi) Makino seed as explant

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[0028] In order to understand the present invention, the present invention will be further described below in conjunction with the examples. The following descriptions are only preferred embodiments of the present invention, and are only used to describe the present invention, and should not be construed as limiting the scope of the present invention. It should be noted that any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention shall be included within the protection scope of the present invention. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0029] In the embodiment, 30g / L sucrose and 2.25g / L phytogel are all contained in the basic medium, the induction medium, the proliferation medium and the rooting medium, and the pH of the above medium is adjusted to 5.8 before sterilization, and the sterilization The conditions were 121°...

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Abstract

The invention belongs to an efficient breeding method capable of taking a Chimonobambusa quadrangularis (Fenzi) Makino seed as an explant. The efficient breeding method comprises the following steps of: selecting the Chimonobambusa quadrangularis (Fenzi) Makino seed which is just harvested at the beginning of May as an explant material, and carrying out preprocessing, disinfecting, inoculating, cluster bud induction, culture and root growth to generate a tissue culture seedling. The efficient breeding method disclosed by the invention fills in a blank that the Chimonobambusa quadrangularis (Fenzi) Makino seed takes the seed as the explant to carry out quick breeding to obtain cluster seedlings, the Chimonobambusa quadrangularis (Fenzi) Makino seed is taken as an explant material, a combination of different growth regulators is combined to design a culture medium suitable for Chimonobambusa quadrangularis (Fenzi) Makino cluster bud induction and root growth, the root growth seedling of the Chimonobambusa quadrangularis (Fenzi) Makino seed can be finished only in about 20 weeks, in addition, a great quantity of cluster seedlings can be obtained in short time, and the defects that Chimonobambusa quadrangularis (Fenzi) Makino is difficult in breeding and traditional stock plant transplantation efficiency is low, transportation cost is high and the like can be effectively overcome.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a high-efficiency breeding method using bamboo seeds as explants. Background technique [0002] The genus Chimonobambusa was established by Japanese scholar Tomitaro Makino in 1914. At that time, there was only one species of Chimonabambusa. Han bamboo is native to my country and Japan, and was introduced to England as early as the 19th century. Bamboo species of the genus Phyllostachys have gradually adapted to cold, humidity-loving and shade-tolerant environments during the long-term evolution process. Therefore, they are mainly distributed in middle and high altitude mountainous areas. Fang bamboo is naturally distributed in the provinces south of the Qinling Mountains and southern Tibet, but the concentrated distribution areas are in Guizhou, Sichuan, Chongqing and Yunnan provinces. It extends southward to Vietnam and Burma, and eastward to Japan. ...

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 王星杨蒙林树燕丁雨龙周必铙卞丽丽徐薪璐
Owner NANJING FORESTRY UNIV
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