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Nannizzia fulva strain and application thereof in degradation of chicken feathers

A technology of Nenitzia chrysogenum and strains, applied in the biological field, can solve problems such as slow degradation, accumulation, and secondary pollution of the ecological environment

Active Publication Date: 2021-06-01
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When keratin waste such as chicken feathers accumulates naturally, it degrades very slowly and easily causes a large amount of accumulation. At the same time, many human or animal pathogenic microorganisms can grow and enrich such fungi using chicken feather keratin as a carbon and nitrogen source, thus causing damage Secondary pollution of ecological environment and harm to human health
The utilization of livestock and poultry feather resources is hindered and wasted in large quantities. Usually, such resources are disposed of by landfill and incineration, but incineration will bring safety hazards to the environment and human health. Therefore, green and healthy methods are used to It is especially important to degrade keratin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Experimental example 1 Degradation test of chicken feather by the strain of Neisseria chrysalis

[0039] Experimental materials and methods

[0040] 1.1 Treatment of chicken feathers: Chicken feathers were collected in a farmer’s poultry slaughterhouse in Huaxi District, Guiyang City. The collected chicken feathers were washed and soaked in tap water for 12 hours, rinsed with running water, autoclaved at 121°C for 30 mins, and dried in an oven at 50°C. spare.

[0041] 1.2 Preparation of fermentation medium

[0042] Chicken feather 10g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.2g, zinc sulfate 0.05g, calcium chloride 0.2g, dilute to 100ml, pH 7.0, autoclave at 121°C for 30 mins, set aside.

[0043] 1.3 Preparation of spore suspension of Nenizia chrysogenum strain

[0044] In the ultra-clean workbench, gently scrape off the hyphae of Neisseria chrysogenum strain GZAC.YNXS.2 cultured on PDA medium at 25°C for 7 days, and transfer it to a sterilized medium ...

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PUM

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Abstract

The invention relates to a Nannizzia fulva strain and an application thereof in degradation of chicken feathers, the morphological characteristics of the strain are as follows: the strain is cultured on a PDA culture medium for 14 days at the temperature of 25 DEG C, the diameter of a bacterial colony is 40mm, and the strain is felty, light brown to grey white to light yellow red, dense in the center, then provided with an annular sparse zone, then provided with a dense annular zone and regular in edge; and the back surface is yellowish-brown to milky white. The hyphae are separated, transparent and smooth, and the width is 1-5 m. The conidia are divided into large conidia and small conidia, the large conidia are rich, the surface of the large conidia is rough, the large conidia is generally divided into 2-6 partitions, the small number of the large conidia is 8 partitions, and the large conidia is in a fusiform to fusiform shape and is 15-58*4.0-10.5 m. The small conidia are rare, most of the small conidia are lateral, smooth and transparent, and are oval to rod-shaped, and the size of the small conidia is 4.0-8.5*1-3.5 m. According to the invention, chicken feathers can be well degraded.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Neisseria chrysogenum, and also relates to the application of the strain of Neisseria chrysogenum in degrading chicken feathers. Background technique [0002] Chicken feather is a raw material rich in keratin. Its crude protein content is more than 80%. It is a kind of organic matter that is difficult to degrade. Long-chain macromolecules, in which hydrogen bonds, disulfide bonds, salt bonds and ester bonds, etc. maintain the spatial configuration of keratin to form a three-dimensional structure, have high stability, and cannot be dissolved under normal conditions, nor can they be digested by general proteases. hydrolyzed. When keratin waste such as chicken feathers accumulates naturally, the degradation is very slow, and it is easy to cause a large amount of accumulation. At the same time, many human or animal pathogenic microorganisms can grow and enrich ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12N1/02A23K10/26A23K10/12C05F1/00C05F17/20C12R1/645
CPCC12N1/02A23K10/26A23K10/12C05F1/005C05F17/20Y02W30/40Y02A40/20
Inventor 韩燕峰邵秋雨张芝元董醇波姚婷梁宗琦
Owner GUIZHOU UNIV
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