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A kind of Nenizum chrysanthemum bacterial strain and its application in degrading chicken feathers

A technology of N. chrysanthemum and strain, applied in the biological field, can solve the problems of waste, secondary pollution of ecological environment, slow degradation and the like

Active Publication Date: 2022-04-15
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When keratin waste such as chicken feathers accumulates naturally, it degrades very slowly and easily causes a large amount of accumulation. At the same time, many human or animal pathogenic microorganisms can grow and enrich such fungi using chicken feather keratin as a carbon and nitrogen source, thus causing damage Secondary pollution of ecological environment and harm to human health
The utilization of livestock and poultry feather resources is hindered and wasted in large quantities. Usually, such resources are disposed of by landfill and incineration, but incineration will bring safety hazards to the environment and human health. Therefore, green and healthy methods are used to It is especially important to degrade keratin

Method used

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Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0038] Experimental example 1 Degradation test of chicken feather by the strain of Neisseria chrysalis

[0039] Experimental materials and methods

[0040] 1.1 Treatment of chicken feathers: Chicken feathers were collected in a farmer’s poultry slaughterhouse in Huaxi District, Guiyang City. The collected chicken feathers were washed and soaked in tap water for 12 hours, rinsed with running water, autoclaved at 121°C for 30 mins, and dried in an oven at 50°C. spare.

[0041] 1.2 Preparation of fermentation medium

[0042] Chicken feather 10g, potassium dihydrogen phosphate 0.5g, sodium chloride 1.2g, zinc sulfate 0.05g, calcium chloride 0.2g, dilute to 100ml, pH 7.0, autoclave at 121°C for 30 mins, set aside.

[0043] 1.3 Preparation of spore suspension of Nenizia chrysogenum strain

[0044] In the ultra-clean workbench, gently scrape off the hyphae of Neisseria chrysogenum strain GZAC.YNXS.2 cultured on PDA medium at 25°C for 7 days, and transfer it to a sterilized medium ...

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PUM

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Abstract

The invention discloses a strain of Neirizia chrysalis and its use in degrading chicken feathers. Its morphological characteristics are as follows: cultivated on PDA medium for 14 days at 25°C, the colony diameter is 40 mm, felt-like, light brown to off-white To light yellowish red, dense in the center, then a ring-shaped sparse band, and then a dense ring band, with regular edges; the back is yellowish-brown to milky white. Hyphae separate, transparent, smooth, 1–5 µm wide. There are two types of conidia: large conidia are abundant, the surface is rough, septated, generally divided into 2-6 compartments, a few have 8 compartments, fusiform to spindle-shaped, 15-58×4.0-10.5µm. Microconidia are rare, mostly lateral, smooth, transparent, elliptic to rod-shaped, 4.0–8.5×1–3.5 µm. The invention can well degrade chicken feathers.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a strain of Neisseria chrysogenum, and also relates to the application of the strain of Neisseria chrysogenum in degrading chicken feathers. Background technique [0002] Chicken feather is a raw material rich in keratin. Its crude protein content is more than 80%. It is a kind of organic matter that is difficult to degrade. Long-chain macromolecules, in which hydrogen bonds, disulfide bonds, salt bonds and ester bonds, etc. maintain the spatial configuration of keratin to form a three-dimensional structure, have high stability, and cannot be dissolved under normal conditions, nor can they be digested by general proteases. hydrolyzed. When keratin waste such as chicken feathers accumulates naturally, the degradation is very slow, and it is easy to cause a large amount of accumulation. At the same time, many human or animal pathogenic microorganisms can grow and enrich su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/02A23K10/26A23K10/12C05F1/00C05F17/20C12R1/645
CPCC12N1/02A23K10/26A23K10/12C05F1/005C05F17/20Y02W30/40Y02A40/20
Inventor 韩燕峰邵秋雨张芝元董醇波姚婷梁宗琦
Owner GUIZHOU UNIV
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