Sensor for detecting plant active small molecules and preparation method

Abstract

The invention relates to a sensor for detecting plant active small molecules and a preparation method, and the sensor comprises: a microneedle array electrode, which is used for detecting the concentration of the plant active small molecules; a transparent flexible substrate, one side of which is provided with a groove for fixing the microneedle array electrode; a transparent flexible film, which is fixed on the other side, far away from the groove, of the transparent flexible substrate; and a plurality of signal conduction circuits, which are arranged between the transparent flexible substrate and the transparent flexible film, wherein each signal conduction circuit penetrates through the transparent flexible substrate and is connected with the microneedle array electrode. The sensor can be attached to the surfaces of leaves, stems and the like of a plant, has dual functions of sensing and fixing, and can enable visible light to irradiate the surface of the plant through the sensor.

Description

technical field [0001] The invention belongs to the field of plant sensors, in particular to a sensor for detecting plant active small molecules and a preparation method. Background technique [0002] Rhizosphere microorganisms are of great significance in the process of plant growth, and quite a variety of rhizosphere microorganisms are beneficial to plant growth. For example, bean vegetables can symbiotically fix nitrogen with the corresponding rhizobia, thereby reducing the input of nitrogen fertilizers, so that bean vegetables not only occupy an important position in vegetable supply, bring huge economic value to farmers, but also contribute to reducing agricultural non-point sources. Pollution makes a substantial contribution. [0003] The different response mechanisms of rhizosphere microorganisms to plant growth promotion and stress will be fed back at levels such as hormones. However, the current standard detection methods are in vitro detection of destructive plan...

AI Technical Summary

Problems solved by technology

However, most of the existing sensor devices are attached to the plant epidermis, which can only detect the information at the plant-environment interface such as the temperature and humidity of the epidermis, but it is difficult to detect the internal biological signals of the stems and leaves of the plant, such as the concentration of active small molecules, etc.

In addition, the existing sensors have the function of penetrating plant epidermis, and they directly contact tissue fluid to realize biosensing. However, most of them are developed based on animal transdermal sensing requirements, and there are still many problems to be solved when they are directly applied to plant sensing For example, the electrode array is not suitable for the thinner leaves and curved stems of plants due to its non-flexibility. For example, animal transdermal sensing requires high biological safety of sensing electrodes, while plant transdermal sensing requires Reliability such as resistance to high temperature / high humidity environment, resistance to direct sunlight, and sensor transparency (does not hinder the photosynthesis of stems and leaves) requires high requirements

Therefore, plant sensors at this stage still have relatively large defects.

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