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Vertical micro-fluidic chip and method for nucleic acid extraction and amplification

A microfluidic chip and nucleic acid technology, applied to laboratory containers, chemical instruments and methods, biochemical equipment and methods, etc., can solve the problems of detection accuracy to be further improved, complex structure, etc., and achieve easy automation, liquid The effect of accurate feeding and compact and simple structure

Pending Publication Date: 2021-05-28
HEMOSMART MEDICAL TECH LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Most of the current microfluidic chips used for nucleic acid detection are horizontal chips, which need to be equipped with many components that are connected to external power sources. The structure is complex and the detection accuracy needs to be further improved.

Method used

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  • Vertical micro-fluidic chip and method for nucleic acid extraction and amplification
  • Vertical micro-fluidic chip and method for nucleic acid extraction and amplification
  • Vertical micro-fluidic chip and method for nucleic acid extraction and amplification

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Embodiment Construction

[0073] The preferred embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings, so that the advantages and features of the present invention can be more easily understood by those skilled in the art. It should be noted here that the descriptions of these embodiments are used to help understand the present invention, but are not intended to limit the present invention.

[0074] As shown in this specification and claims, the terms "comprising" and "comprising" only suggest the inclusion of explicitly identified steps and elements, and these steps and elements do not constitute an exclusive list, and the method or device may also include other steps or elements. As used herein, the term "and / or" includes any combination of one or more of the associated listed items.

[0075] It should be noted that, unless otherwise specified, when a feature is called "fixed" or "connected" to another feature, it can be directly fixed an...

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Abstract

The invention discloses a vertical micro-fluidic chip and a method for nucleic acid extraction and amplification. The vertical micro-fluidic chip comprises a body provided with cavities, wherein the body is also provided with a sample cavity, a reagent cavity, a second amplification cavity and liquid flow channels respectively communicated with the cavities; and vertical micro-fluidic chip further comprises: a first rotary piston which is rotatably arranged in the body, wherein the sample cavity and the reagent cavity are located above the first rotary piston, the first amplification cavity is located below the first rotary piston, a reagent communicating groove capable of communicating a liquid flow channel of the sample cavity with a liquid flow channel of the reagent cavity is formed in the first rotary piston, the first rotary piston is also provided with a nucleic acid transfer groove capable of communicating the liquid flow channel of the sample cavity with the first liquid inlet channel; and a second rotary piston which is rotatably arranged in the body, and an amplification communicating groove capable of communicating the first liquid outlet channel with the liquid flow channel of the second amplification cavity is formed in the second rotary piston. The nucleic acid extraction and amplification device can perform nucleic acid extraction and amplification and is simple in structure and accurate in reagent quantification.

Description

technical field [0001] The invention belongs to the technical field of nucleic acid detection, and relates to a vertical microfluidic chip and method for nucleic acid extraction and amplification. Background technique [0002] Microfluidics is a technology for precisely controlling and manipulating micro-scale fluids. Specifically, it integrates basic operating units such as sample preparation, reaction, separation, and detection in the process of biological, chemical, and medical analysis into a microfluidic device of several square centimeters. On-chip technology that automatically completes the entire analysis process. At present, microfluidic chips have been applied in the field of PCR nucleic acid amplification. For example, Chinese patent CN111760601A discloses a microfluidic chip integrated with a liquid circuit switching valve, which includes a microfluidic chip body, a reagent channel, a liquid circuit switching valve, The waste liquid storage chamber, sample stora...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00C12M1/00C12M1/34C12Q1/686C12Q1/6806
CPCB01L3/5027C12Q1/686C12Q1/6806B01L2200/10C12Q2565/629C12Q2563/107C12Q2523/308
Inventor 颜菁章志伟邹长华
Owner HEMOSMART MEDICAL TECH LTD
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