Human umbilical cord mesenchymal stem cell injection cryopreservation liquid with high multiplication capacity
A technology of proliferative ability and quality stem cells, applied in the field of cell biology, can solve the problems of reduced cell proliferation ability and short cell viability maintenance time, and achieve high survival rate, reduced risk, and reduced content
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[0036] The second aspect of the present invention provides a method for preparing a cryopreserved solution of human umbilical cord mesenchymal stem cell injection with high proliferative capacity, comprising the following steps:
[0037]S1. According to the formula, draw DMSO and add it to the 500mL receiver, then slowly add compound electrolyte injection, dextran 40 glucose injection, sodium chloride injection, glucose injection, serum substitute to it, and shake while adding until mixed;
[0038] S2. Then slowly add the formula amount of human serum albumin, and mix up and down to obtain the cryopreserved solution of the human umbilical cord mesenchymal stem cell injection.
[0039] The method also solves the phenomenon of protein precipitation of human albumin in contact with DMSO.
[0040] In order to reduce the damage caused by DMSO in the freezing solution to cells in a normal temperature environment, in some preferred embodiments, the preparation method of the cryopres...
Embodiment 1
[0051] Example 1 provides a high-proliferation human umbilical cord mesenchymal stem cell injection cryopreservation solution, in parts by volume, its preparation raw materials include 4 parts of DMSO, 50 parts of compound electrolyte injection, and 40 parts of dextran and glucose injection. 10 parts, 5 parts of sodium chloride injection, 5 parts of glucose injection, 30 parts of human albumin, and 5 parts of serum substitute.
[0052] The DMSO is clinical grade DMSO, purchased from WAK-Chemie;
[0053] The compound electrolyte injection was purchased from Hebei Tiancheng Pharmaceutical Co., Ltd.;
[0054] The dextran 40 glucose injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd.;
[0055] The glucose injection was purchased from Jimin Health Management Co., Ltd.;
[0056] The sodium chloride injection was purchased from Guangdong Otsuka Pharmaceutical Co., Ltd.;
[0057] Described human serum albumin was purchased from Okte Fama AB Company;
[0058] The se...
Embodiment 2
[0067] Example 2 provides a cryopreserved solution of human umbilical cord mesenchymal stem cell injection with high proliferative capacity. By volume, its preparation raw materials include 4 parts of DMSO, 45 parts of compound electrolyte injection, and 40 parts of dextran and glucose injection. 10 parts, 5 parts of sodium chloride injection, 5 parts of glucose injection, 30 parts of human albumin, and 5 parts of serum substitute.
[0068] The DMSO is clinical grade DMSO, purchased from WAK-Chemie;
[0069] The compound electrolyte injection was purchased from Hebei Tiancheng Pharmaceutical Co., Ltd.;
[0070] The dextran 40 glucose injection was purchased from Sichuan Kelun Pharmaceutical Co., Ltd.;
[0071] The glucose injection was purchased from Jimin Health Management Co., Ltd.;
[0072] The sodium chloride injection was purchased from Guangdong Otsuka Pharmaceutical Co., Ltd.
[0073] The serum substitute was purchased from Beijing Dakewe Biotechnology Co., Ltd.
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