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miRNA kit for detecting prognosis of lung adenocarcinoma

A detection reagent, a technology for lung adenocarcinoma, which can be used in DNA/RNA fragments, recombinant DNA technology, and microbial determination/inspection, etc., and can solve problems such as unreported miRNA kits

Active Publication Date: 2021-05-07
ZHONGSHAN HOSPITAL FUDAN UNIV
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Problems solved by technology

[0005] There is no report about a miRNA kit for detecting the prognosis of lung adenocarcinoma of the present invention

Method used

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  • miRNA kit for detecting prognosis of lung adenocarcinoma
  • miRNA kit for detecting prognosis of lung adenocarcinoma

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Embodiment 1

[0025]Example 1 Screening and effect verification of genomic

[0026](1) Construction of prognostic score model of lung adenocarcinoma

[0027]1 method

[0028]We first obtain 61 genome knockout of the total genome knockout from the DEPMAP database, 400 genes related to cell proliferation significantly related to cell proliferation. At the same time, we analyzed these gene expression data in the TCGA database to obtain 429 lung adenocarcinoma samples, and further obtained genetically related to cell proliferation and prognosis by single factor Cox regression and Lasso regression, and finally selected six bases : PSMB6, HSPA9, FOLR2, DUT, CDK7, PLK1; Through multi-factor COX regression built score model score = -0.07371 × PSMB6 + 0.23058 × FOLR2 + 0.14739 × DUT + 0.17565 × CDK7 + 0.26704 × PLK1. Based on this scoring model, we calculate the score of each sample. The truncation value is obtained by logistic regression, and the truncation value is 3.539, and the TCGA sample is divided into two ...

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Abstract

The invention relates to the technical field of molecular diagnosis, and particularly relates to a miRNA kit for detecting prognosis of lung adenocarcinoma. The kit comprises a reagent for detecting the expression quantity of a molecular composition. According to the miRNA kit, miRNA related to cell proliferation and prognosis is screened by applying miRNA sequencing and Lasso regression, a prognosis Score is constructed, a corresponding truncation value of the Score in the lung adenocarcinoma is obtained through logistic regression, and the kit can be used for detecting the prognosis of the lung adenocarcinoma. Experiments prove that the method has the advantages of high accuracy and good specificity and has a good application prospect.

Description

Technical field[0001]The present invention relates to the field of molecular diagnosis, in particular, is a miRNA kit for detecting the prognosis of lung adenocarcinoma.Background technique[0002]Lung cancer ranked all tumors first as the most common and most deadly tumors, incidence and mortality. Lung adenocarcinoma is the most common subtype in lung cancer, accounting for more than 40% of lung cancer cases. How to accurately diagnose lung cancer, which is extremely important for the early treatment of patients, and the burden of reducing society is extremely important.[0003]Some of the prognosis of some possible biomarkers (such as gene, etc.) are presented for pre-prognosis of patients with lung adenocarcinoma, but they have not been widely used in clinical practice due to high cost, cumbersome and low accuracy. It is still possible to have a marker with higher sensitivity, specificity and application value, which can be used in the formulation of individual chemotherapy regimens...

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Application Information

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IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/178C12Q2600/158C12Q2600/118
Inventor 卞赟艺毕国澍梁嘉琪胡正阳赵梦男隋启海姚光宇詹成范虹
Owner ZHONGSHAN HOSPITAL FUDAN UNIV
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