Pharmaceutical composition, for preventing or treating sarcopenia, containing salicornia europaea extract

A technology for sarcopenia and Salicornia grass, applied in drug combinations, medical preparations containing active ingredients, muscular system diseases, etc., can solve problems such as unsuitable for long-term treatment, affecting bone formation and regeneration, and masculinization of women , to achieve the effect of complete prevention or treatment

Active Publication Date: 2021-04-30
KOREA UNIV RES & BUSINESS FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But it is not suitable for long-term treatment
Second, as a medical treatment, testosterone or anabolic steroids can be used, but this can lead to virilization in women and side effects such as prostate symptoms in men
In addition, the p21 gene has been reported to inhibit the cell cycle and the proliferation of precursor cells, thereby affecting bone formation and regeneration

Method used

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  • Pharmaceutical composition, for preventing or treating sarcopenia, containing salicornia europaea extract
  • Pharmaceutical composition, for preventing or treating sarcopenia, containing salicornia europaea extract
  • Pharmaceutical composition, for preventing or treating sarcopenia, containing salicornia europaea extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1. Verification of the inhibitory effect against sarcopenia caused by UV irradiation

[0054] C2C12 muscle precursor cells (ATCC, US) were cultured in DMEM containing 10% FBS. At this point, the 3x10 5 Cells were seeded in a 60 pie dish. On the second day after sowing, laminarin and fucoidan were treated separately and left for 1 hour. After 1 hour, the growth media (10% FBS DMEM) was removed and washed with PBS. After removing the PBS, after removing the plate cover from the UV cross-linker (UVP CL-1000), perform 3J / m 2 UV irradiation (0 days). After culturing in a CO2 incubator (Incubator) for 24 hours, the growth medium was removed in the same manner, and PBS was washed, followed by 3J / m 2 UV irradiation (1 day).

[0055] On the next day, harvesting was performed at the same time (2 days), and changes in the expression of myostatin and MURF1 proteins, which are target proteins regulating muscle mass, in cell lysates were detected using Western blotting....

Embodiment 2

[0060] Example 2. Verification of the inhibitory effect against sarcopenia caused by bleomycin

[0061] C2C12 muscle precursor cells (ATCC, US) were cultured in DMEM containing 10% FBS. At this point, the 3x10 5 Cells were seeded in a 60 pie dish and, after seeding, treated with bleomycin and left for 1 hour.

[0062] Next, laminarin and fucoidan were treated separately, and after standing for 1 hour, the growth medium (10% FBS DMEM) was removed and washed with PBS. After washing, Western blotting was used to detect the expression changes of myostatin and MURF1 proteins, which are target proteins regulating muscle mass, in the cell lysates. Comparative analysis was performed using anti-GDF8 (Genetex, GTX32624) as myostatin and β-actin antibody (ENOGene E12-041-4) as protein loading control.

[0063] After RNA was isolated using RNeasy-Mini Kit (Qiagen, US) and cDNA was synthesized using AMV Reverse Transcriptase (Promega, US), the degree of myostatin mRNA expression was con...

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PUM

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Abstract

According to an embodiment of the present invention, provided are a pharmaceutical composition, for preventing or treating sarcopenia, containing a Salicornia europaea extract as an active ingredient, and a food composition, for preventing or alleviating sarcopenia, containing a Salicornia europaea extract as an active ingredient.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for preventing or treating sarcopenia, which comprises an extract of Salicornia viridis as an active ingredient. Background technique [0002] The concept of sarcopenia originated from the "sarcopenia" proposed by Irwin Rosenberg in 1989. It is derived from the Greek and is a combination of "sarco" which means muscle and "penia" which means reduction. Sarcopenia is associated with aging and refers to a loss of muscle strength due to loss of muscle mass. Here, "muscle" refers to skeletal muscle and does not relate to smooth muscle. That is, sarcopenia mainly refers to the loss of skeletal muscle mass distributed in the extremities, which is different from acute diseases such as cachexia (a state of significant muscle loss in the late stage of malignant tumors) and influenza muscle wasting (muscle wasting), or primary muscle disease (primary muscle disease). [0003] The prevalence of osteo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/21A61K31/716A61K31/737A23L33/105A61P21/00
CPCA61K36/21A61K31/716A61K31/737A23L33/105A61P21/00A23V2002/00A23V2200/316
Inventor 金贤洙
Owner KOREA UNIV RES & BUSINESS FOUND
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