Application of preparation for detecting serum IGF1 protein in preparation of colorectal cancer curative effect monitoring reagent

A colorectal cancer, protein technology, applied in the field of biomedicine, can solve the problems of treatment failure, side effects, low drug sensitivity, etc.

Active Publication Date: 2021-04-27
江西省肿瘤医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

A large number of literatures have reported that severe side effects still occur during the treatment of colorectal cancer with chemotherapy or targeted drugs. Repeated high-intensity treatment makes patients less and less sensitive to drugs, and eventually drug resistance (chemo-resistance) occurs leading to treatment failure

Method used

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  • Application of preparation for detecting serum IGF1 protein in preparation of colorectal cancer curative effect monitoring reagent
  • Application of preparation for detecting serum IGF1 protein in preparation of colorectal cancer curative effect monitoring reagent
  • Application of preparation for detecting serum IGF1 protein in preparation of colorectal cancer curative effect monitoring reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1 Differential protein screening based on DIA technology

[0017] 1. Experimental method

[0018] 1. Case information

[0019] A total of 20 patients with stage Ⅲ or stage Ⅳ colorectal cancer were included in this study, and these patients were treated with Sanjie formula and chemotherapy drugs. Quantitative proteomics technology based on full-scan data independent acquisition (DIA) of high-resolution mass spectrometry, and the changes of molecular characteristics of serum samples in 20 patients with stage III or stage IV colorectal cancer before and after treatment with Sanjie formula and chemotherapy drugs . The treatment plan of the patients is shown in Table 1.

[0020] Table 1 Diagnosis and treatment plan of 20 patients with traditional Chinese and western medicine

[0021]

[0022]

[0023] The basic process of the DIA experiment: extract protein from the sample and measure the protein concentration; digest the extracted protein sample into pepti...

Embodiment 2

[0036] PRM experimental verification

[0037] The basic process of the PRM experiment: the first step is to extract the protein from the sample and measure the protein concentration; the second step is to digest the extracted protein sample into peptides by trypsin; the third step is to pass 8 randomly selected proteins through The establishment method of DDA mode to obtain target peptide spectrum information. Fourth, take an equal amount of sample for PRM mode scanning to obtain data; fifth, use Skyline for quantitative analysis.

[0038] The detailed steps are as follows:

[0039] (1) Protein extraction: The serum samples of the above 20 patients with stage III or stage IV colorectal cancer before and after treatment with Sanjie Fang and chemotherapy drugs were randomly mixed into 5 groups, a total of 10 samples. Take 2 μL of each sample for enzyme digestion. For each sample, 1 μL of the mixed pool was used for library construction. Serum and pool samples were digested u...

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Abstract

The invention belongs to the field of biological medicine, and relates to application of a preparation for detecting serum IGF1 protein in preparation of a colorectal cancer curative effect monitoring reagent. According to the application of the invention, the curative effect of the IGF1 protein on predicting the colorectal cancer through a stagnation eliminating formula and chemotherapeutic drugs is evaluated, the AUC value of the IGF1 protein is 1, and the prediction accuracy is as high as 95.5%. The IGF1 protein can be used as a potential biomarker, and a specific, rapid and noninvasive monitoring means is provided for the curative effect and prognosis of advanced colorectal cancer treatment.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically, the invention relates to the application of a preparation for detecting serum IGF1 protein in preparing a reagent for monitoring curative effect of colorectal cancer. Background technique [0002] Colorectal cancer (CRC) is a common malignant tumor in the digestive system. It refers to malignant tumors that occur in the colon (including the ascending colon, transverse colon, descending colon, and sigmoid colon) and the rectum. Its incidence rate ranks third among cancers. Mortality ranks second. More than 50,000 people worldwide die from colorectal cancer every year, and 20% of patients are diagnosed with metastatic colorectal cancer (mCRC) when they are first diagnosed. The incidence of colorectal cancer in my country has shown an obvious upward trend in recent years, and the average age of onset is 41-65 years old, which has seriously endangered and affected the health of our p...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/574
CPCG01N33/74G01N33/57419G01N33/57488G01N2333/65
Inventor 饶军钭方芳郑智熊爱华
Owner 江西省肿瘤医院
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