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Mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity

A nanoparticle and detection method technology, applied in the fields of biochemistry and nanometers, can solve the problems of troublesome pre-treatment analysis technicians, unfavorable rapid analysis, cumbersome operation, etc., and achieve easy promotion and use, simple and fast preparation process, and simple detection process Effect

Active Publication Date: 2021-04-23
FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these methods are cumbersome to operate, require cumbersome pretreatment, specialized analytical technicians, and expensive instruments, which are not conducive to rapid analysis

Method used

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  • Mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity
  • Mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity
  • Mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Mix 0.1 g of heparin with 1 mL of 10 mM potassium hexachloroosmate, then add 48 mL of double distilled water, stir for 30 minutes in the dark, then add 1 mL of 0.2 M sodium borohydride, continue After stirring for 90 minutes, the color of the solution changed from light green to light brown, and 50 mL of heparin-modified osmium nanoparticles (concentration: 38 mg / L) were prepared. Such as figure 2 As shown, A is the osmium nanoparticles prepared without adding heparin ligand, while B is the heparin-modified osmium nanoparticles. After 10 days at room temperature, A has agglomerated sedimentation, while B heparin-modified osmium nanoparticles can maintain good stability .

Embodiment 2

[0040] Add 0.1 mL of heparin-modified osmium nanoparticles prepared in Example 1 (concentration: 38 mg / L) to 0.4 mL, 50 μM mercury ion sample solution. Then, 3300 µL of phosphate buffer solution (pH=6, 20 mM) and 200 µL of 3, 3', 5, 5'-tetramethylbenzidine hydrochloride (2 mM) were added to the mixed solution. After reacting in a water bath at 25 °C for 5 min, the absorbance at the maximum absorption wavelength of 600 nm was measured with a UV-Vis spectrophotometer. image 3 It is a schematic diagram of the detection of mercury ions. After the addition of mercury ions, the absorbance value at the absorption wavelength of 600 nm increases significantly.

Embodiment 3

[0042] Add 0.1 mL of heparin-modified osmium nanoparticles prepared in Example 1 (concentration: 38 mg / L) to 0.4 mL, 50 μM mercury ion sample solution. Then, 3300 µL of phosphate buffer solution (pH=6, 20 mM) and 200 µL of 3, 3', 5, 5'-tetramethylbenzidine hydrochloride (2 mM) were added to the mixed solution. After reacting in a water bath at 25 °C for 5 min, the absorbance at the maximum absorption wavelength of 600 nm was measured with a UV-Vis spectrophotometer. Such as image 3 As shown, the heparin-modified osmium nanoparticles without mercury ions catalyzed 3, 3', 5, 5'-tetramethylbenzidine hydrochloride did not change significantly at the absorption wavelength of 600 nm. ) was added, the absorbance value at the absorption wavelength of 600 nm increased significantly.

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Abstract

The invention discloses a mercury ion colorimetric detection method based on osmium nanoparticle oxidase activity. According to the method of the invention, the heparin-modified osmium nanoparticles and mercury ions interact specifically to simulate the enhancement of oxidase activity, and the heparin-modified osmium nanoparticles sensitized by the mercury ions catalyze and oxidize the 3, 3 ', 5, 5'-tetramethylbenzidine hydrochloride to render color, the absorption value of the chromogenic system at the maximum absorption wavelength of 600 nm is increased along with the increase of the content of the mercury ions, the detection linear range of the mercury ions is 3-40 microns, the detection sensitivity is high, the detection limit of color change observed by naked eyes is 3 microns, and the detection limit of the detection of spectrophotometry is 220 microns. Osmium nanoparticles used for detection are easy and convenient to prepare and easy to obtain and have good stability; the mercury ion detection method constructed on the basis of the material can achieve visual and convenient analysis on mercury ions; and the method has the advantages of being easy and convenient to operate, short in detection time, high in sensitivity, high in specificity and the like and is easy to apply and popularize.

Description

technical field [0001] The invention provides a rapid colorimetric detection method for mercury ions, relates to a colorimetric detection method for mercury ions based on osmium nanoparticle simulated oxidase, and belongs to the fields of biochemistry and nanotechnology. Background technique [0002] Mercury can exist in two forms of simple substance and mercury compound under natural and artificial conditions. Elemental mercury is elemental mercury, also known as metallic mercury. Metallic mercury poisoning is often caused in the form of mercury vapor. Because mercury vapor is highly diffusible and fat-soluble, it enters the alveoli through the respiratory tract and is transported to the whole body through blood circulation. When mercury is discharged into natural water bodies from pollution sources, it can immediately interact with various substances in the water body, including various ions, molecules and complex ligands dissolved in water, organic and inorganic particl...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
CPCG01N21/78G01N21/31Y02A20/20
Inventor 陈伟何少斌邓豪华彭花萍况烨
Owner FUJIAN MEDICAL UNIV
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