Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Mycorrhizal seedling raising method for Rhynchostylis gigantea(Lindl .)Ridl

A technology of Orchid spp. and Orchid spp. is applied in the field of plant cultivation, which can solve the problems of complex cultivation methods and high cultivation costs, and achieve the effects of simple seedling cultivation methods, low seedling cultivation costs, and easy availability of materials.

Inactive Publication Date: 2021-04-02
海南省农业科学院热带园艺研究所
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In recent years, due to a large number of excavation and other reasons, the number of orchids in Hainan has been greatly reduced, so there are more and more methods of raising seedlings for the purpose of protection, but the existing cultivation methods are complicated and costly

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Mycorrhizal seedling raising method for Rhynchostylis gigantea(Lindl .)Ridl
  • Mycorrhizal seedling raising method for Rhynchostylis gigantea(Lindl .)Ridl
  • Mycorrhizal seedling raising method for Rhynchostylis gigantea(Lindl .)Ridl

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The present embodiment provides a method for cultivating mycorrhizal seedlings of orchid hainanensis, which specifically includes the following steps:

[0034] S1. Cultivate Hainan Orchid Orchid Tissue Culture Bottle Seedlings

[0035] ① Seed germination: Select mature pods of orchid hainanensis, wash them with tap water, and wash them with 0.1% HgCl under sterile conditions. 2 Disinfect for 5 minutes, rinse with sterile water several times, take out the seeds, transfer the seeds to the seed germination medium and distribute them evenly, and cultivate them at a temperature of (25±2)°C, an ultraviolet light intensity of 1200-1500lx, and a light of 10h / d Germinated after 30 days to obtain sterile seedling stem segments.

[0036] Specifically, when preparing the seed germination medium, 3 g / L of Huabao No. 1, 0.5 g / L of hydrolyzed milk protein and 0.5 g / L of activated carbon are fully mixed.

[0037] ② Subculture propagation culture: add the stem section of sterile seedl...

Embodiment 2-5

[0063] The main difference between embodiment 2-5 and embodiment 1 is: the seed germination medium consumption, subculture medium consumption etc. are different, and the specific difference of embodiment 1-5 is as shown in table 4 below:

[0064]

[0065] blank control

[0066] Set up a blank control that does not receive the dominant strain (in S6, the tissue culture bottle seedlings obtained by S1 and the pollution-free symbiotic bottle seedlings obtained by S3 are transplanted on the basic cultivation medium), and 3 groups of repetitions are set, with 10 strains for each repetition. Cultivate in an artificial incubator (temperature 25°C, photoperiod 12 / 12h, sterilization 1600lx for standby).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of plant cultivation, and particularly discloses a mycorrhizal seedling raising method for Rhynchostylis gigantea(Lindl .)Ridl. The method comprises the following steps that S1, Rhynchostylis gigantea(Lindl .)Ridl. tissue culture bottle seedlings are cultivated; S2, a solid microbial agent is prepared, an activated bacterial cake is obtained through hyphae of a dominant strain, the sequence of the dominant strain is SEQ ID NO: 1; S3, pollution-free symbiotic bottle seedlings are obtained, specifically, the activated bacterial cake obtained in the S2is put into the tissue culture bottle seedlings of the Rhynchostylis gigantea(Lindl .)Ridl. tissue culture bottle seedlings cultured in the S1, so as to obtain the pollution-free symbiotic bottle seedlings; S4, a liquid microbial agent is prepared, specifically, the activated bacterial cake obtained in the S2 is made into a liquid microbial agent; S4, preparing a liquid microbial agent; S5, a culture medium is prepared by adopting the liquid microbial agent prepared in the step S4; and S6, the tissue culture bottle seedlings are transplanted. The seedling raising method provided by the invention can realize industrial seedling raising of the Rhynchostylis gigantea(Lindl .)Ridl. seedlings, the seedling raising cost is low, and the seedling raising method is simple.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a method for mycorrhizal seedling cultivation of orchid hainanensis. Background technique [0002] Rhynchostylis gigantea (Lindl.) Ridl is an epiphytic orchid belonging to the Orchidaceae family Rhynchostylis gigantea (Lindl.) Ridl. It is only distributed in Hainan Province in China, and it is a rare wild species under national second-level protection. . The flowering period of Hainan Orchid Orchid is from January to February, which coincides with the Spring Festival in China and some Asian countries. The flowers have a strong fragrance and a long flowering period, and are very popular among consumers. Hainan Orchid Orchid has excellent characteristics such as large number of flowers, thick flowers, good meat texture, strong heat resistance, easy hybridization and good affinity, and its ornamental value, economic value and research value are very high. [...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 任军方李栋梁蔡嘉慧杨珺陈宣周新成
Owner 海南省农业科学院热带园艺研究所
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com