Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Rooting method of eucalyptol type oil camphor tree tissue cultured buds

A technology of eucalyptol and camphor tree, applied in the field of plant reproduction, can solve the problems of difficult rooting of sub-generation shoots, long root initiation time, easy blackening of the root system, etc., and achieves rooting rate and transplanting survival rate improvement, rooting rate High and shortened rooting cycle

Inactive Publication Date: 2017-04-05
GUANGXI FORESTRY RES INST
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problems of the difficulty in rooting of shoots of the existing dual-purpose eucalyptol type camphor tree tissue, low rooting rate, long initial rooting time, few hair roots, and easy blackening of the root system, etc., to provide a Rooting method of tissue culture buds of eucalyptol type camphor tree with fast rooting and good effect

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Select the eucalyptol-type oil camphor tree sub-bottle seedlings that have been cultured for 30-35 days in tissue culture, and after sterilizing the surface of the bottle, in the sterile space of the ultra-clean workbench, select sub-generation buds that grow robustly and have a height of ≥ 2cm single bud, cut at 1-2mm below the node, remove the basal leaves and petioles, insert L-cysteine ​​20mg / L sterile solution at 0.4-0.5 cm from the bottom of the trimmed single bud stem. Soak for 15-20min for rooting pretreatment.

[0023] Insert the pretreated single shoot vertically into the rooting medium, the formula of the rooting medium is: modified LS medium+IBA 1.0 mg / L+ABT 1 1.5 mg / L + polyvinylpyrrolidone 30 mg / L + sucrose 15 g / L + agar powder 5.8 g / L. The formula of the modified LS medium described therein is: NH 4 NO 3 650 mg / L+KNO 3 1900mg / L+CaCl 2 ·2H 2 O 400mg / L+MgSO 4. ·7H 2 O 370 mg / L+KH 2 PO 4 170 mg / L+H 3 BO 3 6.2 mg / L+MnSO 4. ·4H 2 O 22.3 mg / L+ZnSO...

Embodiment 2

[0026] Select the eucalyptol-type oil camphor tree sub-bottle seedlings that have been cultured for 30-35 days in tissue culture, and after sterilizing the surface of the bottle, in the sterile space of the ultra-clean workbench, select sub-generation buds that grow robustly and have a height of ≥ 2cm single bud, cut at 1-2mm below the node, remove the basal leaves and petioles, insert L-cysteine ​​20mg / L sterile solution at 0.4-0.5 cm from the bottom of the trimmed single bud stem. Soak for 18min and perform rooting pretreatment.

[0027] Insert the pretreated single shoot vertically into the rooting medium, the formula of the rooting medium is: modified LS medium+IBA 1.5 mg / L+ABT 11.5 mg / L + polyvinylpyrrolidone 30 mg / L + sucrose 15 g / L + agar powder 5.8 g / L. The formula of the modified LS medium described therein is: NH 4 NO 3 650 mg / L+KNO 3 1900mg / L+CaCl 2 ·2H 2 O 400mg / L+MgSO 4. ·7H 2 O 370 mg / L+KH 2 PO 4 170 mg / L+H 3 BO 3 6.2 mg / L+MnSO 4. ·4H 2 O 22.3 mg / L+...

Embodiment 3

[0030] Select the eucalyptol-type oil camphor tree sub-bottle seedlings that have been cultured for 30-35 days in tissue culture, and after sterilizing the surface of the bottle, in the sterile space of the ultra-clean workbench, select sub-generation buds that grow robustly and have a height of ≥ 2cm single bud, cut at 1-2mm below the node, remove the basal leaves and petioles, insert L-cysteine ​​20mg / L sterile solution at 0.4-0.5 cm from the bottom of the trimmed single bud stem. Soak for 20min and conduct rooting pretreatment.

[0031] Insert the pretreated single shoot vertically into the rooting medium, the formula of the rooting medium is: modified LS medium+IBA 2.0 mg / L+ABT 1 2.0 mg / L + polyvinylpyrrolidone 30 mg / L + sucrose 15 g / L + agar powder 5.8 g / L. The formula of the modified LS medium described therein is: NH 4 NO 3 650 mg / L+KNO 3 1900mg / L+CaCl 2 ·2H 2 O 400mg / L+MgSO 4. ·7H 2 O 370 mg / L+KH 2 PO 4 170 mg / L+H 3 BO 3 6.2 mg / L+MnSO 4. ·4H 2 O 22.3 mg / L...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a rooting method of eucalyptol type oil camphor tree tissue cultured buds. The method comprises the following steps: selecting eucalyptol type oil camphor tree subcultured buds obtained after 30-35 d bud strengthening culture in tissue culture, shearing strong single buds with the height being not less than 2 cm from subcultured bud clusteres, inserting the single buds into an aseptic solution of L-cysteine, immersing the single buds to preprocess the single buds, vertically inserting the preprocessed single buds into a rooting medium, carrying out rooting culture in specific light and temperature environment, moving the rooted buds to a seedling growing shed with natural environment conditions, and hardening seedlings to obtain eucalyptol type oil camphor tree seedlings. The method solves the problems of difficult rooting, low rooting rate, long rooting time, small root number and easy blackening of roots of the eucalyptus oil-type camphor tree subcultured buds, and has the advantages of short rooting period, high rooting rate, large root quantity, good quality, high transplanting survival rate of tissue-cultured and rooted seedlings, realization of tissue culture industrial seedling growth, provision of high quality seedlings for construction of a natural perfume artificial clonal forest, and good economic, social and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant reproduction, relates to the tissue culture technology of camphor tree, and in particular relates to a rooting method for the tissue culture buds of eucalyptol-type camphor tree. Background technique [0002] Eucalyptus oil (cineole, C 10 H 18 Cinnamomum camphora (L.) Presl, also known as camphor, is a national class II protected plant, mainly distributed in Jiangxi, Hunan and Guangdong in China. , Fujian, Hainan, Guangxi, Sichuan, Taiwan and other places, as well as foreign countries such as Vietnam, South Korea and Japan. Grows in hills and low mountains. It is a multi-purpose excellent tree species that is used for skidding, oil and landscape gardening. Eucalyptol is a colorless, transparent, volatile oily liquid with a camphor smell and a spicy cool taste. It mainly exists in the volatile oils of aromatic plants such as eucalyptus and camphor, and has been widely used in spices, food, medici...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 蔡玲韦颖文李开祥安家成杨素华黄宏喜覃杰
Owner GUANGXI FORESTRY RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com