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Development method of pine immune resistance inducer

An immune inducer, pine technology, applied in the field of research and development of pine immune inducer, can solve the problems of high preparation cost, low efficacy, difficult absorption of polypeptides, etc., and achieve the effect of improving disease resistance and reducing preparation cost

Inactive Publication Date: 2021-03-30
杭州益森键生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It can significantly improve the disease resistance and stress resistance of pine trees, and solve the problems of existing peptides that are not easy to be absorbed, have low efficacy and high production cost

Method used

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  • Development method of pine immune resistance inducer

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Effect test

Embodiment 1

[0029] The present invention is a kind of development method of pine immune inducer, comprising the following steps:

[0030] Step 1: Degrade soybean polypeptide powder and filter to obtain the first raw material;

[0031] Step 2: Dissolving 1-n-dodecylazepan-2-one with absolute ethanol to obtain the second raw material, 1-n-dodecylazepan-2-one and the anhydrous The weight ratio of ethanol is 1:3-1:5;

[0032] Step 3: Formulating fatty alcohol polyoxyethylene ether into an aqueous solution to obtain a third raw material, the weight ratio of fatty alcohol polyoxyethylene ether to water is 1:4-1:6;

[0033] Step 4: adding the first raw material, the second raw material and the third raw material into water and mixing in sequence, and heating the mixed solution in a water bath at 35-50° C. for 10-12 hours under the assistance of ultrasonic waves to obtain the pine immune inducer.

[0034] Soybean polypeptide powder is prepared by the following method:

[0035] Put the soybean ...

Embodiment 2

[0045] Indoor pine immune inducer permeability test: get two groups of pine trees to get samples, one group is 3-5 parts, choose one group to inject the pine immune inducer prepared by the present invention, and at the same time, other immune inducer in the market Inject the antibiotic into another group of pine trees to take samples, and after standing for 24 hours, observe the drug absorption situation of the samples taken by the two groups of pine trees through an optical microscope, and draw a conclusion by observing the penetration of the samples taken by the two groups of pine trees. The penetration rate of the pine immune inducer is as high as more than 85%, while the penetration rate of the general pine immune inducer reaches 60%. Therefore, the pine immune inducer of the present invention is more easily absorbed and utilized through the xylem of the plant.

[0046]Through our research, we found that after the soybean enzyme is hydrolyzed into peptides, the liquid is cu...

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Abstract

The invention discloses a development method of a pine immune resistance inducer, and relates to the technical field of plant immune resistance inducers. The development method comprises the followingsteps: degrading and filtering soybean polypeptide powder to obtain a first raw material; dissolving 1-n-dodecyl azacycloheptane 2-ketone with absolute ethyl alcohol to obtain a second raw material;preparing fatty alcohol-polyoxyethylene ether into an aqueous solution to obtain a third raw material, adding the first raw material, the second raw material and the third raw material sequentially into water to be mixed, heating the mixed solution for 10-12 hours in a water bath at the temperature of 35-50 DEG C under the assistance of ultrasonic waves so that the pine immune resistance inducer is obtained. Soybean polypeptide powder is degraded and filtered and then mixed with the second raw material and the third raw material, the mixture conveniently penetrates through xylem of plants to be efficiently absorbed and utilized, soybean protein is used for preparing the soybean polypeptide powder, and the preparation cost is reduced; meanwhile, the plant immune resistance inducer preparedby taking the soybean polypeptide powder as a key raw material can remarkably improve the disease resistance, stress resistance and yield increase capacity of plants.

Description

technical field [0001] The invention belongs to the technical field of plant immune inducers, in particular to a preparation method of a pine immune inducer. Background technique [0002] Plant immune inducers, also called plant vaccines, are a new field of vaccine engineering technology after human vaccines and animal vaccines, and are also a popular research direction for the creation of biopesticides in the world. When plants are stimulated by the outside world or under adverse conditions, they can produce immune responses by regulating their own defense and metabolic systems. This defense response or immune resistance response of plants can delay or alleviate the occurrence and development of diseases in plants and reduce chemical stress. The use of pesticides and chemical fertilizers reduces the residues of agricultural products. Plant immune inducers have no direct killing effect on crop diseases and insect pests, but are induced or activated by exogenous organisms or ...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K1/34A01N37/46A01P21/00
CPCC12P21/06C07K1/34A01N37/46
Inventor 孙学书王永春
Owner 杭州益森键生物科技有限公司
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